摘要
目的验证脱细胞肝脏细胞外基质的生物相容性，优化研制的肝脏组织工程支架及培养条件。方法将人肝癌细胞系——HepG2细胞以1×105细胞／cm。的密度种植在六种不同脱细胞肝脏细胞外基质支架上，分别在静置和动态条件下（10r／min）培养12d。结果种植在六种不同脱细胞肝脏细胞外基质支架上的HepG2细胞都显示了明显的细胞增殖。HepG2细胞种植12d后，与其他方法比较，水洗法处理的细胞外基质支架细胞增殖明显增加（P〈0．05）。动态培养条件下，HepG2在细胞外基质表面均匀生长，形成5—6层，并且细胞向基质内部生长4—6层。结论我们研究制备的肝脏细胞外基质具有良好的生物相容性，优化了脱细胞方法和培养条件。
Objective To validate the biocompatibility of decellularized specific extracellular matrix （ECM） derived from poroine liver and optimize the existed liver tissue scaffolds and culture conditions. Methods HepG2 cells were cultured in 6 different liver tissue scaffolds with density of 1 x 105/era2, fol- lowed by 12 d static and dynamic culture （ 10 r/min）. The proliferation of HepG2 ceils as well as the cell- matrix interaction were studied. Results All the HepG2 cells cultured in 6 different liver tissue scaffolds of extracellular matrix presented with predominant cell proliferation. After 12 d culture of HepG2 cells, the cell proliferation of extracellular matrix scaffolds which processed with water rinse presented with more significant increase （P 〈 0.05） than any other processing approaches. Under dynamic culture, HepG2 cells were de- scribed as uniform growth in the surface of extracellular matrix developed 5-6 layers. Besides, these ceils grew into matrix for 4-6 layers. Conclusion In this study, we verified the biocompatibility of liver ECM, and optimized the management approaches of decellularization and culture condition.
出处
《中华肝胆外科杂志》
CAS
CSCD
北大核心
2016年第9期626-629,共4页
Chinese Journal of Hepatobiliary Surgery
基金
国家自然科学基金项目（81571825）
北京市自然科学基金项目（7152063）
北京市教育委员会科技发展计划项目（KM2016100250017）
关键词
肝脏
猪
细胞外基质
肝脏组织工程支架
动态培养
Liver, porcine
Extracellular matrix （ ECM ）
Liver tissue engineering scaffold
Dynamic culture
