摘要
目的:建立高效液相色谱法同时测定降糖类中成药中9种非法添加的化学成分。方法:采用C18色谱柱,以甲醇-0.1 mol·L^(-1)乙酸铵溶液梯度洗脱,检测波长235 nm,柱温35℃,同时进行全波长扫描(190~400 nm),并对可疑成分进行质谱确认。结果:9种对照品在各自浓度范围内,呈现良好的线性关系(r≥0.999 9),精密度、稳定性、检出限、定量限、回收率均符合规范,并检出降糖宁中非法添加了格列本脲。结论:该方法简便快速准确,适用于上述降糖类化学成分的检测。
Objective： To establish an HPLC method for simultaneous determination of 9 illegally added anti-diabetic chemical components in Chinese patent medicines. Method： The compounds were separated on C18 column,with methanol-0. 1 mol·L- 1ammonium acetate solution as the mobile phase for gradient elution. The detection wavelength was set at 235 nm and the column temperature was 35 ℃,with the flow rate of 1. 0 mol·L-1.Full wavelength scanning was from 190 nm to 400 nm. Suspicious ingredients were confirmed by mass spectrometry. Result： All 9 components had good linear relationship within their respective concentration range（ r≥0. 999 9）,and their precision,stability,limit of detection,limit of quantitation and recovery rate met the standards. Illegally added glyburide was detected in Jiangtangning. Conclusion： The method is simple,accurate,and suitable for the determination of 9 illegally added anti-diabetic chemical components above.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第19期66-69,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江苏高校品牌专业建设工程项目(PPZY2015A097)
