摘要
目的研究5-Aza-d C对肺癌细胞系中的去甲基化的作用。方法对正常肺细胞以及5-Aza-d C分组处理的A 549、NCI-H 520甲基化特异性PCR和RT-PCR定量检测。结果未处理的A 549和NCI-H 520细胞中TFPI-2表现出完全甲基化特异性。而经过5-Aza-d C处理过的肺癌细胞系细胞则表现出不完全甲基化状态,并呈现出量效关系。A 549和NCI-H 520细胞在经5-Aza-d C处理后TFPI-2基因m RNA表达均明显高于未处理组,差异具有统计学意义(P<0.05);并呈现出量效关系。结论 5-Aza-d C对肺癌细胞中的TFPI-2基因具有去甲基化作用,并具有量效关系。
Objective To study the effect of 5-Aza-dC on the methylation of lung cancer cell lines. Methods Quantitative detection of A 549, NCI-H 520 methylation specific PCR and RT-PCR in normal lung cells and 5-Aza-dC group. Results TFPI-2 in untreated A 549 and NCI-H 520 cells showed complete methylation specificity. The 5-Aza-dC treated lung cancer cell lines showed incomplete methylation status, and showed a dose effect relationship. After 5-Aza-dC treatment, A 549 and NCI-H 520 cells were significantly higher than the untreated group, the difference was statistically significant （P〈0.05）, and showed a dose effect relationship. Conclusion 5-Aza-dC has a role in the methylation of TFPI-2 gene in lung cancer cells, and has dose effect relationship.
出处
《当代医学》
2016年第29期10-11,共2页
Contemporary Medicine
