摘要
目的:通过固定循经取穴配伍以不同部位选穴进行对比研究,以糖尿病胃轻瘫(diabetic gastroparesis,DGP)大鼠为观察对象,研究不同按部选穴针刺治疗对DGP大鼠Rho A/ROCK信号的表达差异,探讨按部选穴是影响腧穴配伍效应的主要影响因素.方法:将60只♂SPF级SD大鼠,适应性喂养1 w k后,随机分为空白对照组、模型组、足三里+中脘组、足三里+内关组、足三里+非经非穴组,每组12只.除空白对照组12只外,其余48只大鼠运用链脲佐菌素腹腔注射造糖尿病模型,普通喂养8 wk后建立DGP大鼠模型,针刺治疗4 wk,于13 w k末墨汁灌胃后处死,取胃窦组织.运用Western blot检测胃窦平滑肌组织Ras同源物基因组成员A(Ras homolog gene family,member A,Rho A)、Rho蛋白相关卷曲螺旋激酶(Rho-associatedc,oiled-coil containing protein kinase,ROCK)、肌球蛋白磷酸酶靶亚单位1(myosin phosphatase target subunit 1,MYPT1)、p-MYPT1蛋白的表达量;应用免疫组织化学检测胃窦平滑肌组织Rho A蛋白平均灰度值改变.结果:与空白对照组相比,模型组的小肠推进率、胃窦平滑肌组织Rho A、ROCK、MYPT1、p-MYPT1蛋白的表达量明显降低(P<0.01),胃窦平滑肌组织R h o A灰度值表达升高(P<0.05).与模型组相比,足三里+中脘组、足三里+内关组、足三里+非经非穴组的小肠推进率和胃窦平滑肌组织Rho A、ROCK、MYPT1、p-MYPT1蛋白的表达量明显升高(P<0.05),胃窦平滑肌组织Rho A灰度值表达均具有降低的趋势(P<0.05).与足三里+中脘组相比,足三里+内关、足三里+非经非穴组胃窦平滑肌组织R h o A、ROCK、MYPT1、p-MYPT1蛋白的表达量降低(P<0.05),胃窦平滑肌组织Rho A灰度值表达升高(P<0.05).在治疗期间,与模型组相比,足三里+中脘组的饮食量明显降低(P<0.05).结论:针刺能通过上调Rho A/ROCK信号的表达来促进胃平滑肌收缩,改善DGP的症状;证实按部选穴是影响腧穴配伍效应的重要因素,且配伍局部穴明
AIM： To observe the effect of acupuncture at different acupoints on the RhoA/ROCK signaling pathway in the gastric antrum of diabetic gastroparesis （DGP） rats. METHODS： Sixty SD rats were randomly divided into a normal group, a model group, a Zusanli ＋ Zhongwan group, a Zusanli ＋ Neiguan, and a Zusanli ＋ non-acupoint group, with 12 rats in each group. DGP was indueced by intraperitoneal injection of streptozotocin, followed by feeding for 8 wk. After 4 wk of treatment, the gastrointestinal transit rate was measured with black ink. The expression of RhoA protein and ROCK protein was detected by imrnunohistochemistry and Western blot. RESULTS： Compared with the normal group, the model group showed a significant reduction in gastrointestinal transit rate and expression of RhoA, ROCK, MYPT1, and p-MYPT1 in gastric antral smooth muscle tissue and a significant increase in RhoA expression （P 〈 0.01）, suggesting that DGP was successfully induced. Compared with the model group, the gastrointestinal transit rate and expression of RhoA, ROCK, MYPT1 and p-MYPT1 in gastric antrum tissue significantly increased in the Zusanli ＋ Zhongwan, Zusanli ＋ Neiguan and Zusanli ＋ non-acupoint groups （P 〈 0.05）. RhoA, ROCK, MYPT1 and p-MYPT1 expression was significantly higher in the Zusanli ＋ Zhongwan group than in the Zusanli ＋ Neiguan and Zusanli ＋ non-acupoint groups （P 〈 0.05）. RhoA expression in gastric antral smooth muscle tissue was significantly higher in the Zusanli ＋ non-acupoint groups Neiguan and Zusanli ＋ than in the Zusanli ＋ Zhongwan group （P 〈 0.05）. The dietary intake was significantly reduced in the Zusanli ＋ Zhongwan group compared with the model group during the treatment period （P 〈 0.05）. CONCLUSION： Acupoint selection is an important factor to influence the effect of acupoint compatibility. Combination of local acupoints is obviously superior to the compatibility of distal acupoints and non-acupoint.
出处
《世界华人消化杂志》
CAS
2016年第23期3508-3516,共9页
World Chinese Journal of Digestology
基金
国家重点基础研究计划基金资助项目
No.2014CB543102~~
