摘要
目的建立蛋鸡骨质疏松模型,研究蛋鸡骨质疏松,为人类骨质疏松的研究提供参考。方法用低钙日粮(钙含量1.5%),连续60d诱发笼养蛋鸡骨质疏松症,并设立对照组(钙含量3.7%),分光光度法检测血清中钙、磷、碱性磷酸酶和抗酒石酸酸性磷酸酶的含量;称量蛋鸡股骨和胫骨的重量;游标卡尺测量骨骼的长度、宽度;万能材料试验机进行三点弯曲实验检测骨骼的强度;并制作观察骨组织切片进行HE染色,分析骨切片上成骨细胞、破骨细胞和骨细胞的变化。结果研究发现与对照组相比,低钙日粮导致了血清中碱性磷酸酶含量显著性增加,血清钙含量显著性下降,蛋鸡股骨和胫骨重量显著性变轻,宽度显著性变窄,骨强度显著性降低。病理切片结果显示,与对照组相比,低钙组蛋鸡股骨远端骨切片中成骨细胞和破骨细胞含量均增加,但骨细胞含量减少。结论连续60d给蛋鸡饲喂低钙日粮可成功建立蛋鸡骨质疏松模型,且该模型为高转化型骨质疏松。
Objective Cage layer osteoporosis is a common bone disease,which leads to a decline of eggshell quantity,an increase of fracture rate,paralysis,and even death in hens.The disease has a big negative effect on the modern chicken industry.The chicken osteoporosis model not only plays an important role in research of cage layer osteoporosis,but also in the human osteoporosis.Methods In order to establish the model,the low calcium diet which only included 1.5%of calcium was used for inducing the cage layer osteoporosis.3.7%of calcium was used as control.The serum levels of calcium,phosphates,alkaline phosphates（ALP）,and tartrate resistant acid phosphates（TRACP） were detected using spectrophotometric method.The weight,width,and length of the tibia and femur were measured.The strength of the tibia and femur were measured by 3-point bending test using an universal material testing machine.Bone slices were HE-stained for the analysis of osteoblasts,osteoclasts,and osteocytes on the slices.Results The experiment results showed that compared with those in the control group,the hens in low calcium group had an increase of serum ALP and a decrease of calcium.The weight of the tibia and femur in low calcium group was lighter than that in control group.The width of the tibia and femur in low calcium diet group was narrower.And the strength of the bone was weaker.Pathological section of the distal femur results showed that the bone slices of the hens in low calcium group had more osteoclasts and osteoblasts but less bone cells than in the control group.Conclusion The experiment suggests that the chicken osteoporosis model can be induced by low calcium diet intake for 60 days.The model is a high bone-turnover model.
作者
刘秀云
孙玉玺
李西林
江莎
LIU Xiuyun SUN Yuxi LI Xilin JIANG Sha(College of Animal Science and Technology, Southwest University,Chongqing 400715, China)
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2016年第9期1087-1091,共5页
Chinese Journal of Osteoporosis
基金
西南大学本科生科技创新基金(20152802001)
中央高校基本科研业务费专项资金(XDJK2016E038
DJK2014C123)
关键词
笼养蛋鸡
骨质疏松
高转化型
碱性磷酸酶
病理切片
Cage hens
Osteoporosis
High bone turnover
Alkaline phosphatase
Pathological slices
