摘要
目的观察复方番石榴制剂对Hep G2细胞胰岛素抵抗(IR)的改善作用,及对α-淀粉酶和α-葡萄糖苷酶抑制作用。方法用高浓度胰岛素诱导Hep G2细胞建立胰岛素抵抗细胞模型,用葡萄糖氧化酶法检测复方番石榴制剂对IR-Hep G2细胞葡萄糖的消耗量;分别以可溶性淀粉和4-硝基酚-α-D-吡喃葡萄糖苷(PNPG)为底物测定复方番石榴制剂对α-淀粉酶和α-葡萄糖苷酶活性的抑制率。结果与模型组比较,复方番石榴制剂(62.5,250,500μg·m L^(-1))显著增加了IR-Hep G2细胞对葡萄糖的消耗量(P<0.05,P<0.01)。在实验剂量范围内,复方番石榴制剂对α-淀粉酶和α-葡萄糖苷酶抑制作用均随浓度升高而抑制作用增加,IC50值分别为0.79 mg·m L^(-1)和0.48 mg·m L^(-1)。结论复方番石榴制剂可促进IR-Hep G2细胞对葡萄糖的消耗,改善Hep G2细胞对胰岛素的抵抗状态,并能体外抑制α-淀粉酶和α-葡萄糖苷酶活性。
Objective To investigate the effect of compound Fructus Psidii Guajavae preparation on insulin resistance（IR） in HepG2 ceils and its inhibitory effect on ot-amylase and ct-glucosidase. Methods IR-HepG2 cell model wasinduced by high concentration of insulin, and the glucose consumption in IR-HepG2 ceils affected by compoundFructus Psidii Guajavae preparation was detected by glucose oxidase method. The inhibitory effects of compoundFructus Psidii βGuajavae preparation on the activity of a-amylase and ot-glucosidase were examined by usingP-nitrophenyl-β- galactoside （PNPG） and starch as substrate respectively. Results Compared with the model group,compound Fructus Psidii Guajavae preparation at 62.5, 250, 500 μg·m L-1 significantly increased the glucoseconsumption of IR-HepG2 cells （P 〈 0.05 or P 〈 0.01 ）. In the range of experimental doses, the inhibition ofct-amylase and ct-glucosidase was increased with the increase of concentration of compound Fructus Psidii Guajavaepreparation, and the ICso values were 0.79 μg·m -1 and 0.48 mg·mL-1. Conclusion Compound Fructus PsidiiGuajavae preparation can promote the glucose consumption of IR-HepG2 ceils, improve the insulin resistance ofHepG2 ceils, and inhibit the activity of tx-amylase and ct-glucosidase in vitro.
作者
戴卫波
李乐愚
梅全喜
DAI Weibo LI Leyu MEI Quanxi(Zhongshan Hospital Affiliated to Guangzhou University of Chinese Medicine, Zhongshan 528400 Guangdong, Chin)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2016年第5期655-660,共6页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省中医药局立项资助项目(No.20141282)
广东省中山市科技局重点资助项目(No.2014A1FC004)
关键词
复方番石榴制剂
胰岛素抵抗
Α-淀粉酶
Α-葡萄糖苷酶
糖代谢
Compound Fructus Psidii Guajavae preparation
insulin resistance
ct -amylase
eL-glucosidase
glycometabolism
