摘要
目的：探讨人宫颈癌基因（HCCR）在原发性肝细胞癌患者中的表达及其临床意义。方法采用实时荧光定量逆转录聚合酶链反应（FQ-RT-PCR）技术检测60例原发性肝细胞癌和肝硬化、正常健康体检者血清中HCCR RNA 的表达，并分析其与临床病理特征的关系。结果外周血单核细胞（PBMC）中，肝癌患者 HCCR RNA的表达强度为0.515±0.092，肝硬化患者 HCCR RNA 的表达强度为0.348±0.064，而正常健康体检者 HCCR RNA无表达，差异均有统计学意义（P 〈0.05）。FQ-RT-PCR 结果显示肝癌患者检出率最高，达83.33%，其次是肝硬化组（8.33%），而健康人对照组检出率为0。肝癌患者检出率与甲胎蛋白（AFP）表达量、门静脉癌栓、肿瘤分化程度、临床 TNM 分期等临床参数密切相关（P 〈0.05）。结论原发性肝细胞癌患者、肝硬化患者 PBMC 中 HCCR mRNA表达明显增高，HCCR 基因可能参与了原发性肝细胞癌的发生与发展，其与原发性肝细胞癌的恶变演进有一定的相关性。
Objective To investigate the expressional level of human cervical cancer oncogene （HCCR）in primary hepatocellular carcinoma（ PHC）and its clinical significance. Methods HCCR RNA in serum were detected by fluorescent quantitation reverse transcription polymerase chain reaction （FQ-RT-PCR）in 60 patients with HCC,hepatocirrhosis and normal health examination,and its correla-tion with the clinical pathological features was analyzed. Results The relative expression levels of HC-CR RNA in the peripheral blood mononuclear（PBMC）of PHC patients were 0. 515 ± 0. 092,and which in the hepatocirrhosis patients were 0. 348 ± 0. 064,while HCCR RNA expression was not detected in the normal health examination,the difference was significant（P 〈 0. 05）. FQ-RT-PCR results showed that the detection rate of PHC was the highest（83. 33% ）,the second was hepatocirrhosis group（8. 33% ）, while that of the normal health examination group was 0. 00% ,and the expression of HCCR RNA was strongly correlated with AFP expression quantity,portal thrombosis,tumor differentiation degree and TNM stage（P 〈 0. 05）. Conclusions The HCCR mRNA expressional levels of PBMC in patients with PHC,hepatocirrhosis increase significantly,which show that HCCR may participate in the occurrence of PHC and is correlated with the evolution of malignant change in the PHC.
出处
《中国实用医刊》
2016年第17期27-30,共4页
Chinese Journal of Practical Medicine
基金
湖南省教育厅科研项目（12C1239）
关键词
人宫颈癌基因
原发性肝细胞癌
肝硬化
Human cervical cancer oncogene
Primary hepatocellular carcinoma
Hepatocirrhosis
