摘要
模式生物斑马鱼已广泛应用于肾发育和疾病的研究。斑马鱼肾结构、功能、细胞组成类型均与人类肾相似,此外,斑马鱼肾发育迅速,基因编辑技术成熟,易于进行高通量基因功能筛查研究,是肾发育和疾病研究的理想模式生物。足细胞损伤是肾小球疾病发生发展的关键环节,是导致蛋白尿、肾小球硬化及肾功能进行性恶化的重要病理基础。文中将简述斑马鱼肾结构功能,斑马鱼肾滤过功能评估方法,斑马鱼足细胞损伤模型,以及斑马鱼应用于足细胞研究的最新进展。
Zebrafish have been emerging as a valuable model for kidney research, including podocyte biology, nephrogenesis and kidney disease. The zebrafish kidney has similar structure, common function and the same cell types with human kidney. Rapid development and feasible genetic editing approaches in zebrafish provide an ideal model for high-throughput gene screening involved in kidney disease and developmental process. Loss or injury of podocytes is the key mechanism, which can lead to proteinuria, glomerulo- sclerosis and renal failure. Here, we introduce structure and function of zebrafish kidney, filtration estimating assays, podocytes injury model and the highlights of recent zebrafish podocytes studies.
出处
《医学研究生学报》
CAS
北大核心
2016年第9期976-981,共6页
Journal of Medical Postgraduates
基金
国家自然科学基金(81470943)
南京军区南京总医院科研基金(2016036)
关键词
斑马鱼
肾发育
足细胞
＂1 Zebrafish
Kidney development
Podocytes
