摘要
向凡纳滨对虾高位池(面积均为0.067 m^2)养殖环境中添加甘蔗渣悬浮颗粒与芽孢杆菌,设置不同梯度放养量,分别放养6万与16万尾虾苗,各处理组为对照组6、蔗渣和菌组6,对照组16、蔗渣和菌组16,通过测定水质、细菌数量和对虾生长等指标,评估对养殖环境和对虾生长的影响,实验结果表明:各组生物絮团含量最大值分别为1.1 m L/L,1.0 m L/L,0.4m L/L,0.4 m L/L,都维持在较低水平,各养殖密度条件下添加蔗渣和菌组都大于相应对照组,这说明甘蔗渣悬浮颗粒和芽孢杆菌的添加,对水体中生物絮团的形成有一定的促进作用。各养殖密度下蔗渣和菌组水体中芽孢杆菌数量维持在10~4 cfu/m L左右,而对照组都则维持在10~2 cfu/m L左右,蔗渣和菌组分别大于相应对照组,而弧菌数量在实验后期要低于相应对照组,原因可能是甘蔗渣悬浮颗粒的添加,为芽孢杆菌提供了大量的附着基,有效增加了水体中芽孢杆菌数量,抑制了弧菌生长。收获时,蔗渣和菌组6对虾体重为9.09±0.43 g,对照组6为8.19±0.36 g,显著大于对照组(P<0.05);蔗渣和菌组16(10.42±0.55g)也显著大于对照组16(8.33±0.51 g)(P<0.05);蔗渣和菌组6的产量为325 kg,比对照组6高100 kg;蔗渣和菌16组最终产量为381 kg,比对照组16高276.5 kg。研究表明:甘蔗渣悬浮颗粒和芽孢杆菌的添加,能促进生物絮团的形成,增加和维持水体中芽孢杆菌等有益菌的数量,改善养殖环境,促进对虾生长,提高对虾产量和成活率。
Added suspended bagasse and Bacillus sp. to Litopenaeus vannamei high-place culture pond（the area was 0.067 hm^2）. Set up two different density gradient（60 000 and 160 000）, the treatment groups were control group 6, added bagasse with Bacillus sp. 6, control group 16, added bagasse with Bacillus sp. 16 group.To estimate the effect of addition of suspended bagasse with Bacillus sp. on the environment of culture pond and the growth of shrimp, the quality of water, the quantity of bacteria and the growth of shrimp index were detected. The result suggested that the concentration of bio-floccules were 1.1 m L/L, 1.0 m L/L, 0.4 m L/L, 0.4m L/L respectively, each group maintained in low level, and added bagasse with Bacillus sp. groups were higher than that of control group, which indicated that the addition of suspended bagasse and Bacillus sp. could promote the formation of bio-floccules. The quantity of Bacillus sp. of added bagasse with Bacillus sp. Groups were 10~3-10~4 cfu/m L, which was higher than that of corresponding control groups（10~2 cfu/m L）, while the quantity of vibrio during the latter stage of the culture were in contrast. The reason may be that the addition of suspended bagasse provide lots of substrata for Bacillus sp., which could increase the quantity of Bacillus sp. and inhibit vibrio. The average weight of added bagasse with Bacillus sp.6（9.09±0.43 g） and added bagasse with Bacillus sp. 16（10.42±0.55 g） were significantly higher than that of control group 6（8.19±0.36 g） and control group 16（10.42 ±0.55 g） respectively when harvest（P0.05）. The yield of added bagasse with Bacillus sp. 6 group were 325 kg, which was higher than control group 6 by 100 kg, the final yield of added bagasse with Bacillus sp. 16 group were 381 kg, while that of control group 16 were 104.5 kg. The addition of suspended bagasse with bacillus played a positive role in improving the culture environment, which could increase and maintain the quantity of beneficial bacteria, promote the for
出处
《浙江海洋学院学报（自然科学版）》
CAS
2016年第2期132-136,154,共6页
Journal of Zhejiang Ocean University(Natural Science Edition)
基金
国家科技支撑项目(2011BAD13B10)
宿迁市经济鱼类繁育公共服务平台(M201423)
