摘要
MYB转录因子是在真核生物细胞内广泛存在的一类转录因子蛋白,在植物的生长发育、代谢调节、抗病抗逆、以及激素介导的信号路径等方面都发挥着重要的作用。本研究从海岛棉品种海7124中克隆得到一个MYB转录因子基因,根据序列同源性和进化分析,将其命名为GbMYB60。该基因序列长990 bp,编码一个36.9 k D的R2R3类MYB转录因子蛋白。GbMYB60蛋白被特异地定位于植物细胞核。GbMYB60基因的表达水平较低,在真叶中优势表达,根系中表达量最低。该基因受甘露醇、Na Cl、低温、高温等非生物逆境,以及脱落酸、乙烯利、茉莉酸甲酯和水杨酸等植物激素的诱导上调表达。利用病毒诱导的基因沉默技术在海岛棉中干涉GbMYB60发现,降低GbMYB60基因的表达水平,使棉花幼苗对高盐胁迫的耐受性降低;但GbMYB60干涉的植株在甘露醇溶液的处理下与对照植株并无显著的抗性差异。
MYB transcription factors are conserved proteins in all eukaryotic cell, which play important roles in plant growth, development, metabolism, abiotic and biotic stress resistance, as well as phytohormone-mediated signal transduction. In this research, a MYB gene was isolated from the sea-island cotton cultivar Hai 7124. This gene was named GbMYB60 based on the sequence similarity search and phylogenetic analysis. The full-length of GbMYB60 coding sequence is 990 bp, and GbMYB60 encodes a 36.9 k D R2R3-type MYB protein, which is specifically located in nucleus of plant cell. GbMYB60 was preferentially expressed in leaf and induced by abiotic stresses（such as salt, mannitol, cold, and heat） and phytohormones（abscisic acid, ethephon, methyl jasmonate, and salicylic acid） treatments, but the general expression of GbMYB60 was low in all tissues. Salt and mannitol tolerances were analyzed in control and GbMYB60 silenced-cotton generated by virus-induced gene silencing system, and the results showed that GbMYB60 positively regulated cotton tolerance to salt but not to mannitol.
出处
《作物学报》
CAS
CSCD
北大核心
2016年第9期1342-1351,共10页
Acta Agronomica Sinica
基金
国家重点基础研究发展计划项目(2012CB1143001)
棉花生物学国家重点实验室开放课题(CB2015A31)
河南省教育厅项目(15A180028
15A180029)资助~~
