摘要
以红腰豆黄酮粗提物为原料,比较6种树脂对红腰豆黄酮的静态吸附和解吸性能,筛选出XDA-1进行分离纯化实验,并考察红腰豆黄酮纯提物对食用油脂(猪油和花生油)的抗氧化效果。结果表明:最佳的分离条件为上样液质量浓度1.5 mg/m L,吸附流速2 BV/h,上样液p H值6,上样量3 BV,洗脱速度2 BV/h,乙醇体积分数为60%洗脱,洗脱量3 BV。在此工艺条件下,黄酮得率及纯度分别为73.86%和22.65%。红腰豆黄酮提取物可有效延缓油脂氧化,且对猪油的抗氧化效果优于花生油。
Using crude flavonoids extracted from red kidney beans as the raw materials, the adsorption and desorption efficiencies of flavonoids with six different macroporous resins were compared,and XDA - 1 resin was selected as the most efficient adsorbent as it had good adsorption and desorption capacity for flavonoids from red kidney beans. The antioxidation effect of flavonoids extract from red kidney beans on edible oils and fats （lard and peanut oil） was investigated. The results showed that the optimal separation condition was： sample liquid concentration 1.5 mg/mL, adsorption flow rate 2 BV/h , sample pH value 6 , sample volume 3BV, elution speed 2 BV/h , and ethanol volume fraction 6 0 % , elution volume 3BV. Under the optimal separation condition the flavonoids yield and purity were 73. 86% and 22. 6 5 % , respectively. The flavonoids extract can effectively defer the lipid oxidation, and moreover its antioxidanteffect on lard was better than that on peanut oil.
出处
《粮油食品科技》
2016年第5期29-34,共6页
Science and Technology of Cereals,Oils and Foods
基金
龙岩学院产学研项目(LC2015011)
关键词
红腰豆
黄酮
分离
大孔树脂
抗氧化
red kidney bean
flavone
separation
croporous resin
antioxidation
