摘要
以玉米(Zea mays L.)品种郑单958为试验材料,利用免疫印迹等技术,确定干旱高温复合胁迫条件下,Ca^(2+)/CaM对sHSP26的表达、叶绿体抗氧化防护酶活性的影响。结果显示:(1)高温、高温干旱复合胁迫下sHSP26的表达量明显高于对照和干旱,经CaCl_2预处理后能显著增强sHSP26的表达;(2)经CaCl_2预处理后,叶绿体内超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GR)和抗坏血酸过氧化物酶(APX)活性显著增加,而经Ca^(2+)螯合剂EGTA及CaM抑制剂TFP和W7处理后,这些抗氧化防护酶活性显著下降。本研究表明,在干旱高温复合胁迫条件下适宜浓度的Ca^(2+)能够增强sHSP26的表达及提高叶绿体抗氧化防护酶活性,从而对叶绿体起到保护作用,提高植物耐热性。
Taking maize variety （Zea mays L. ） Zhengdan 958 as experimental material, we used technologies like immunoblotting and determined the effect of Ca^2＋/CAM on sHSP26 expression, antioxidant defense system under the combination of drought and heat stress. The main results showed that： （1） the expression of sHSP26 under heat stress or combined drought and heat stress is significantly higher than that under control and drought, and after pretreatment with CaCl2 , sHSP26 expression is much higher than that of the untreated group ; （2） after pretreatment with CaC12 , superoxide dismutase （ SOD）, glutathione reductase （ GR）, ascorbate peroxidase （APX） activity was significant- ly increased, but after pretreatment with Ca^2＋ chelator EGTA and CaM antagonists TFP and W7, the activity of these enzymes significantly decreased. These results showed that Ca^2 ＋/CaM enhanced the antioxidant defense system and the expression of sHSP26, which protected maize chloroplasts from the damage caused by combined drought and heat stress, and enhanced maize endurance to stress.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2016年第4期447-452,共6页
Journal of Henan Agricultural University
基金
国家自然科学基金项目(31171470)
河南省高校科技创新人才支持计划(13HASTIT001ABA)
