摘要
目的观察职业性哮喘大鼠模型激发后嗜酸性粒细胞与嗜酸性粒细胞趋化因子（Eotaxin）和白细胞介素-5（IL-5）以及骨髓CD34＋／CCR3＋和CD34＋／IL-5Ra＋变化的关系，探讨大鼠CD34＋祖细胞分化机制，并观察中药温肾阳干预的影响。方法将40只造模后健康雄性SD大鼠随机分为模型组、醋酸泼尼松干预组、温肾]gH＋l／2醋酸泼尼松干预组和温肾阳干预组，每组10只，并设空白对照组，分别给予生理盐水（20ml／kg），醋酸泼尼松（8．22mg／kg）、温肾阳醋酸泼尼松和温肾阳（20g／kg）干预。通过细胞计数、免疫组化、ELISA、流式细胞技术和原位杂交等方法，分别观察肺组织嗜酸性粒细胞、Eotaxin表达，外周血嗜酸性粒细胞，血清Eotaxin和IL-5含量，骨髓CD34＋／CCR3＋和CD34＋／IL-5Ra＋表达。结果模型组肺组织嗜酸性粒细胞、Eotaxin表达，外周血嗜酸性粒细胞，血清Eotaxin和IL-5含量，骨髓CD34＋／CCR3’和CD34＋／IL．5Ra嚷达均较对照组明显增高，差异有统计学意义（P〈0．01）。醋酸泼尼松干预组、温肾阳＋1／2醋酸泼尼松干预组和温肾阳干预组肺组织嗜酸性粒细胞、Eotaxin表达，外周血嗜酸性粒细胞，血清Eotaxin和IL-5含量，骨髓CD34＋／CCR3＋和CD34＋／IL．5Ra嚷达均较模型组明显下降，差异有统计学意义（P〈0．01或P〈0．05），且温肾阳＋1／2醋酸泼尼松干预组肺组织和外周血嗜酸性粒细胞，血清Eotaxin和IL-5含量以及骨髓CD34＋／CCR3＋和CD34＋／IL-5Ra＋表达较醋酸泼尼松干预组的和温肾阳干预组下降明显，差异有统计学意义（P〈O．05）；肺组织中嗜酸性粒细胞分别与外周血清的Eotaxin和IL-5含量及骨髓CD34＋／CCR3和CD34＋／IL-5Rd表达水平呈正相关（0．9666、0．9829、0．9142、0．8874）。结论职业性哮喘模型大鼠抗原激发后肺内嗜酸性粒细胞增高与骨髓CD34＋／CCR3＋和CD34＋�
Objective To investigate the mechanism of CD34＋ progenitor cell differentiation in rat by observing the change relations between the eosinophils （EOS） and the content of Eotaxin and IL-5 in blood and the CD34＋/CCR3＋, CD34＋/IL-5Ra＋ in bone marrow after occupational asthma （OA） model rats are simulated, and to observe the effect of WTKYD Trraitional Chinese Medicine intervention. Methods A total of 40 healthy male SD model rats （200 - 250 g weight） were randomly divided into model contrast Group, prednisone acetate intervention Group, WTKYD＋I/2 prednisone acetate intervention Group and WTKYD intervention Group, 10 in each group, and set a Group for blank contrast. Give them saline （20 ml/kg）, prednisone acetate （8.22 mg/kg）, WTKYD （20g/kg） ＋1/2 prednisone acetate （4.11 mg/kg） and WTKYD （20 g/kg） intervention respectively. By means of cell count, immunohistochemical, ELISA, flow cytometry technique, situ hybridization and so on, to observe EOS and the expression of Eotaxin in lung tissue, the EOS in peripheral blood, the content of Eotaxin and IL-5 in blood as well as the expression of CD34＋/CCR3＋ and CD34＋/IL-5Ra＋ in bone marrow respectively. Results The number of EOS, the content of Eotaxin and IL-5, the expression of CD34＋/CCR3＋ and CD34＋/IL- 5Ra＋ in Model Contrast Group were higher in Blank Contrast Group, the difference was statistically significant （P〈0.01）, while they were lower in medical intervention Groups when comparing to Model Contrast Group, the difference was statistically significant （P〈0.01 or P〈0.05）, and the above items in WTKYD ＋1/2 Prednisone Acetate Intervention Group were even lower than in Prednisone Acetate Intervention Group and WTKYD Intervention Group, the difference was statistically significant （P〈0.05）. EOS in lung tissue is highly positive related to the content of Eotaxin and IL-5 in peripheral blood as well as the expression of CD34＋/CCR3 and CD34＋/IL-SRa in bone marrow （0.9666,0.9829,0.9142, 0
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
2016年第9期653-658,共6页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
浙江省中医药科技计划项目（2012ZB122）
杭州市卫生科技计划项目（20128008）
关键词
哮喘
植物
药用
祖细胞
Asthma
Plants, medicinal
Stemcells
