摘要
最近的研究结果证实慢性乙型肝炎患者血清中存在的HBVRNA为3．5kb的前基因组RNA（pgRNA），这些pgRNA存在于形态结构类似于丹颗粒的病毒样颗粒中。由于pgRNA只能由位于感染的肝细胞核内的病毒共价闭合环状DNA（cccDNA），且其产生过程不受核苷（酸）类抗病毒药物的影响，因此血清病毒RNA应能够反映接受治疗的患者肝细胞内cccDNA的存在及转录活性。据此推测，血清病毒RNA可作为一个潜在的指导核昔（酸）类似物安全停药的指标。基于小样本停药队列研究的结果显示停药点血清中HBVRNA的确与慢性乙型肝炎患者停药后的病毒学反弹相关．
Our recent studies confirmed that the HBV RNAs present in the serum of chronic hepatitis B （CHB） patients are pregenomic RNAs （pgRNAs） with a size of 3.5 kb. These pgRNAs are located in virus- like particles whose morphological structure is similar to that of Dan particles. Since pgRNAs can only be transcribed from the covalently closed circular DNA （cccDNA） located in the nuclear of infected hepatocytes, and the production of pgRNAs is not affected by nucleos（t）ide analogues （NUCs）, the presence of viral RNA in serum can reflect the presence of cccDNA in hepatocytes and its transcriptional activity in patients treated with medication. It can be inferred that serum viral RNA can be used as a potential index for safe withdrawal of NUCs. Our results based on a small-sample cohort show that the presence of HBV RNA in serum at the time of drugs withdrawal is indeed associated with virological rebound after drug withdrawal.
出处
《中华肝脏病杂志》
CAS
CSCD
北大核心
2016年第9期641-642,共2页
Chinese Journal of Hepatology
