摘要
目的通过观察有氧运动对兔膝骨关节炎（OA）软骨中Ⅱ型胶原和糖胺聚糖（GAG）含量及软骨细胞凋亡率的影响，探讨有氧运动对OA的预防作用及其可能的作用机制。方法将新西兰大白兔20只按随机数字表法分为A、B、C、D四组，每组5只大白兔。入组后，A组笼内自由活动9周；B组笼内自由活动4周；C组接受有氧运动，训练速度为0．5km／h，每周3次，每次20min，训练4周；D组亦接受有氧运动，训练速度为1．5km／h，每周5次，每次20min，共4周。B、c、D3组均于活动或训练4周后，采用木瓜蛋白酶法建立OA模型，1周后经磁共振成像（MRI）检查，均确认造模成功，然后笼内自由活动4周。入组9周后，将4组大白兔处死，采用Mankin评分比较各组问关节软骨损伤程度，并对软骨Ⅱ型胶原的表达、软骨GAG的含量以及软骨细胞凋亡率进行检测。结果人组9周后，B、c、D3组大白兔的Mankin评分、Ⅱ型胶原和GAG含量均显著低于A组，差异均有统计学意义（P〈O．05），B组的Mankin评分、Ⅱ型胶原和GAG含量亦显著低于D组，差异均有统计学意义（P〈O．05）。人组9周后，A组的软骨细胞凋亡率为（3．45±18）％，显著低于B、C、D3组，差异均有统计学意义（P〈O．05），且c、D2组的软骨细胞凋亡率亦显著低于与B组，差异均有统计学意义（P〈0．05）。结论有氧运动可能通过抑制骨性关节炎关节软骨基质中Ⅱ型胶原和GAG总量的减少预防兔膝关节软骨的退变，其作用机制可能与软骨细胞凋亡的减少有关。
Objective To observe the effect of aerobic exercise of different intensities on type Ⅱ collagen, glycosaminoglycan （GAG） and chondrocyte apoptosis in rabbits modeling knee osteoarthritis （OA）, so as to explore the preventive effect and its possible mechanism. Methods Twenty healthy New Zealand white rabbits were ran- domly divided into Groups A, B, C and D, each of 5. Group A was allowed free activity in a cage for 9 weeks. Group B was allowed free activity for 4 weeks, then an OA model was established using papain and confirmed via MRI 1 week later, Another 4 weeks of free activity were then allowed. Groups C and group D were given running training for 20 minutes a day at 0.5 km/h, 3 times a week, and then 20 minutes a day at 1.5 km/h, 5 days a week on a treadmill for 4 weeks. Nine weeks later, all 4 groups of rabbits were killed and the articular cartilage damage of each group was compared using Mankin scoring, and expression of type Ⅱ collagen, GAG content and chondrocyte apoptosis in the cartilage. Results After the intervention, the average Mankin score, expression of type lI collagen and GAG con- tent of groups B, C and D were significantly lower than those of group A, and all of those values in group B were sig- nificantly lower than those of group D. After 9 weeks tbe chondrocyte apoptosis rate of group A was significantly lower than that of the other groups, and that of groups C and D was significantly lower than that of group B. Conclusion Aerobic exercise may prevent knee articular cartilage degeneration through inhibiting reduction in the amount of type II collagen and GAG in the cartilage matrix. It may be related to decreasing the chondrocyte apoptosis.
出处
《中华物理医学与康复杂志》
CAS
CSCD
北大核心
2016年第8期561-565,共5页
Chinese Journal of Physical Medicine and Rehabilitation
关键词
有氧运动
骨关节炎
Ⅱ型胶原
糖胺聚糖
软骨细胞凋亡
Aerobic exercise
Osteoarthritis
Type I1 collagen
Glycosaminoglycan
Chondrocytes
Apoptosis
