摘要
在合成牛血清白蛋白（BSA）修饰的金纳米团簇（金簇）的基础上,将荧光素异硫氰酸酯（FITC）与金簇偶联,构建一种新型比率型p H荧光探针。当体系p H值在4.0~10.0之间变化时,FITC的荧光强度逐渐增加,而金簇的荧光强度基本保持不变。在p H为5.5~8.0之间,该探针对于p H具有良好的线性关系,线性方程为Y=1.72X-8.8,相对标准偏差为0.994,同时该探针在p H为6.0~9.0之间还具有良好的可逆性。此外,将此p H荧光探针与PK15细胞共培养,通过MTT实验证明该比率型p H荧光探针对细胞几乎没有毒性。
A red-emitting gold nanoclusters was prepared by using the bovine serum albumin（BSA） and a ratiometric fluorescent pH probe was prepared by using the BSA-capped AuNCs and FITC.Results showed that the fluorescence intensity of FITC was gradually enhanced with pH changing from acid to alkali condition,while the fluorescence intensity of AuNCs was constant.The probe displayed a fine linear relationship towards pH, with the corresponding linear regression equation of Y= 1.72X-8.8 and the relative standard deviation of 0. 994 when the pH changed from 5.5 to 8.0.The probe had good reversibili- ty when the pH was between 6.0 and 9.0.In addition, the PK15 cells were cultivated with the pH probe. Result of MTT assay showed that the pH probe was almost no toxicity to cells.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2016年第3期77-82,共6页
Journal of Huazhong Agricultural University
基金
国家自然科学基金项目(21305049
31372439)
