摘要
目的观察右美托咪定对兔单肺通气期间肺组织损伤及Toll样受体4(TLR4)、核因子κB p65(NF-κB p65)、细胞间黏附分子1(ICAM-1)mRNA表达的影响。方法 30只新西兰白兔随机分为3组:双肺通气组(T组)、单肺通气组(O组)、右美托咪定联合单肺通气组(D-O组),每组10只。T组行双肺通气3.5 h,O组、D-O组于双肺通气30 min后行右单肺通气3 h。D-O组于气管切开前静脉泵注右美托咪定1μg/kg(10 min泵注完毕),随后以1μg/(kg·h)的速度持续泵注;T组、O组则持续泵注等容量的生理盐水。各组于实验结束后处死动物,取下左侧肺组织,进行肺组织湿/干(W/D)质量比测量;采用HE染色,光镜下观察双侧肺组织病理学改变;并采用实时定量PCR法检测左侧肺组织TLR4、NF-κB p65、ICAM-1 mRNA的表达。结果与T组比较,O组和D-O组左肺W/D比均显著增加;与O组比较,D-O组左肺W/D比显著降低。与T组比较,O组、D-O组肺组织病理学损伤程度均明显加重,但D-O组肺损伤程度明显较O组减轻。与T组比较,O组肺组织中TLR4、NF-κB p65、ICAM-1 mRNA的表达均显著升高,而D-O组肺组织中TLR4、NF-κB p65、ICAM-1 mRNA表达则显著低于O组。结论右美托咪定通过抑制TLR4、NF-κB p65 mRNA的水平,减轻炎症和兔单肺通气所致的肺组织损伤。
Objective To investigate the effect of dexmedetomidine on lung injury and the expressions of Toll-like receptor 4 （TLR4）, nuclear factor KB p65 （NF-κB 1065） and intercellular adhesion molecular 1 （ICAM-1） mRNA during one-lung ventilation （OLV） in rabbits. Methods Thirty healthy New Zealand white rabbits were randomly divided into three groups （n = 10 in each group） ： two-lung ventilation （TLV） group （group T）, OLV group （group O）, dexmedetomidine used during OLV group （group D-O）. The rabbits in group T were treated with TLV for 3.5 hours, while in group O and group D-O, the rabbits were ventilated through dght lung for 3 hours following 30-minute TLV. In group D-O, dexmedetomidine （1 μg/kg） were given intravenously for 10 minutes before tracheostomy, followed by intravenous infusion at the rate of 1 μg/（ kg ·h）. Equal volume of normal saline was given in group O and group T as controls. At the end of the experiment, rabbits were sacrificed and lung tissues were collected. The pulmonary wet/dry mass （W/D） ratio was calculated and the pathological changes of the lungs were observed using HE staining under a light microscope. The expressions of TLR4, NF-κB p65, ICAM-1 mRNA were analyzed by real-time quantitative PCR. Results W/D ratio of left lung tissues in group O and group D-O were significantly higher as compared with group T. However, W/D ratio in group D-O was obviously lower than that in group O. Compared with group T, both group O and group D-O showed much more serious morphological damage in the lung, and such lung injury was less obvious in group D-O than in group O. The expressions of TLR4, NF-κB 1065, ICAM-1 mRNA increased significantly in group O as compared with group T, and such enhancement was ameliorated by dexmedetomidineas observed in group D-O. Condusion Dexmedetomidine might inhibit inflammatory responses and attenuate OLV-induced lung injury in rabbits, possibly by suppressing the expressions of TLR4 and NF-κB p65 mRNA.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2016年第9期1197-1201,共5页
Chinese Journal of Cellular and Molecular Immunology
