摘要
在LED白光(W)和紫外光(UV-A)两种不同光条件下,研究培养液中添加富氢水(HRW)对萝卜芽苗菜生长、下胚轴中花青苷含量及抗氧化能力的影响,并检测了花青苷合成过程中关键结构基因的表达量变化。结果表明,与白光相比,紫外光处理显著抑制了萝卜芽苗菜的生长。不同连续光照时间(3、12、24、36h)下,紫外光处理的萝卜芽苗菜下胚轴花青苷含量均显著高于白光处理;连续光照处理24h和36h时,UV-A+HRW处理下花青苷含量显著高于其他处理,表明UV-A有利于花青苷的合成,而HRW可进一步提高UV-A对花青苷合成的诱导作用;各连续光照时间下,DPPH自由基清除能力的变化趋势与花青苷含量基本一致。与白光相比,紫外光处理可明显提高花青苷合成关键基因的表达;UV-A+HRW处理下,PAL、CHS、CHI、F3H、DFR、LDOX和ANS的表达量均显著高于其他处理,表明UV-A+HRW处理下,上述基因的上调对花青苷含量的提高起到主要调控作用。
Effects of hydrogen-rich water （ HRW ） on growth, anthoeyanin content and antioxidant capacity of radish sprouts cultured under 2 different light conditions of LED white light and UV-A irradiation were studied, and changes in transcript levels of anthocyanin biosynthesis-related genes were examined. The results showed that compared with the white light treatment, UV-A treatment could significantly depress the growth of radish sprouts. And under different continuous irradiation times （ 3, 12, 24 and 36 hours ）, the anthocyanin content of radish sprouts treated with UV-A were significantly higher than that treated with white light. Compared with other treatments, UV-A＋HRW treatment could significantly increase anthocyanin content after 24 hours and 36 hours continuous irradiation, indicating that UV-A irradiation was favorable for anthocyanin biosynthesis, while, HRW could further enhance the abduction of this biosynthesis. And the changing trend of DPPH free radical scavenging ability were consistent with anthocyanin content under various continuous lighting times. Compared with the white light treatment, UV-A treatment could obviously increase the transcript levels of anthocyanin biosynthesis-related genes, the transcript levels of PAL, CHS, CHI, F3H, DFR, LDOX and ANS, treated by UV-A＋HRW were all remarkably higher than that of the other treatments, indicating that under UV-A＋HRW treatment, the upregulation of those genes was playing leading role in increasing anthocyanin contents.
出处
《中国蔬菜》
北大核心
2016年第9期40-46,共7页
China Vegetables
基金
国家自然科学基金项目(31572169)
江苏省农业科技自主创新资金项目〔CX(15)1040〕
