摘要
[目的]提高动物源性成分检测效率,建立PCR-核酸试纸条快速检测技术体系。[方法]针对物种特异性DNA序列,设计并标记引物;建立并优化PCR反应体系;利用通用核酸试纸条进行结果判读。[结果]试验表明,PCR-核酸试纸条特异性高;绝对灵敏度为0.001 3 ng/μL,混合样品,实际灵敏度为0.01%;加工处理样品,实际灵敏度为0.1%;最低检测限检出率为100%,稳定性良好。[结论]综上,PCR-核酸试纸条方法是一种既有PCR反应的高灵敏度,又具有免疫学检测的特异性好的特点,同时操作简便的快速检测方法。
[Objective] In order to improve the efficiency of detecting ingredients of animal origin,the PCR-nucleic acid strip rapid detection technology system was established. [Method]In view of the species specific DNA sequences,primers were designed and marked; the PCR reaction system was established and optimized; the results were interpretated by the nucleic acid strip. [Result] The results showed that： the PCR- nucleic acid strip is high specificity; absolute sensitivity is 0. 001 3 ng / μL; the actual sensitivity in mixed samples is 0. 01%; the actual sensitivity in processing sample is 0. 1%; the detection rate of minimum detection limit is 100%,the stability is good. [Conclusion] To sum up,the high sensitivity as PCR and high specfticity as immunology made the PCR- chromatography strip valuable for the rapid detection method.
出处
《安徽农业科学》
CAS
2016年第20期83-85,117,共4页
Journal of Anhui Agricultural Sciences
基金
国家质检总局课题项目(2014IK105)
关键词
核酸试纸条
动物源
食品安全
Nucleic acid test paper
Animal origin
Food safety
