摘要
目的 :验证脂肪酰胺水解酶(fatty acid amide hydrolase,FAAH)在小鼠精原干细胞(spermatogonial stem cell,SSCs)中的表达。方法:构建稳定的小鼠SSCs培养平台,Western blot法检测FAAH在小鼠各组织脏器和SSCs的表达,免疫荧光法检测FAAH在睾丸组织及SSCs中的定位情况。结果:Western blot结果表明FAAH在睾丸组织高表达。免疫荧光显示FAAH定位于小鼠SSCs的细胞膜并且与已知的分选标记神经胶质细胞源性的神经营养因子(GDNF)家族受体α1(GDNF family receptor alpha-1,GFRα1)共定位。结论:FAAH可作为一个新的SSCs分选富集的表面分子标记,为进一步研究FAAH在精子发生的作用打下基础。
Objective：To validate fatty acid amide hydrolase （FAAH） expression in mouse spermatogonial stem cells （SSCs）. Methods： We established a stable mouse SSCs line in vitro and verified FAAH expression in various tissues of mice and the cell line by Western blotting assay. Immunofluoreseence was performed to detect FAAH location in the testis and SSCs. Results： Western blotting assay showed that FAAH was highly expressed in testieular tissues. Double-immunofluorescent staining showed that FAAH located on the membrane of mouse SSCs and co-localized with SSCs separation marker, GDNF family receptor alpha-1 （GFRα1）. Conclusion： FAAH is a new candidate separation surface marker of SSCs, which provides a basis for the research of FAAH function in spermatogenesis.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2016年第7期778-782,共5页
Journal of Nanjing Medical University(Natural Sciences)
基金
国家自然科学基金面上项目(31271537)
关键词
精原干细胞
脂肪酰胺水解酶
细胞分选
spermatogonial stem cells
fatty acid amide hydrolase （FAAH）
cell sorting
