摘要
目的研究漆树酸(anacardic acid,AA)对人卵巢癌细胞的作用,并初步探讨其作用机制。方法选取两种卵巢癌细胞(SKOV3和HO8910),设置对照组及漆树酸终浓度为2.5μmol/L、5μmol/L、10μmol/L、15μmol/L的漆树酸组,采用MTT法检测漆树酸对细胞增殖的影响;Transwell检测漆树酸对卵巢癌细胞迁移侵袭能力的影响;鬼笔环肽染色检测细胞侵袭伪足形成并进行细胞划痕修复实验;RT-PCR和Western blotting检测漆树酸10μmol/L、15μmol/L组PI3K、VEGF及Caspase3的m RNA及蛋白表达水平变化。结果随AA浓度增加,SKOV3和HO8910细胞株24 h光密度值均呈增加趋势(P<0.05)。AA作用24 h后,10μmol/L、15μmol/L组的SKOV3和HO8910与对照组相比,增殖活性明显增强(P<0.05),与对照组比较,PI3K和VEGF的m RNA及蛋白表达明显上调(P<0.05),而Caspase3的m RNA及蛋白表达降低(P<0.05)。结论漆树酸可能通过调节PI3K、VEGF、Caspase3信号通路,促进卵巢癌细胞增殖,诱导细胞侵袭迁移,侵袭伪足形成。
Objective To investigate the effect of anacardic acid（AA） on ovarian cancer cell lines and its molecular mechanism. Methods Two groups of ovarian carcinoma cell lines（SKOV3 and HO8910） were exposed to AA（2.5, 5, 10 and 15μmol/L）. MTT method was employed to evaluate the cell proliferation effects of AA, and Transwell was performed to detect the migration and invasion of AA on ovarian cancer cell. Cells invading pseudopodia formation and scratch repair experiments were tested by Ghost pen cyclic peptide dyeing. Reverse transcription polymerase chain reaction（RT-PCR） and western blotting were used to assess the m RNA and protein expression levels of Phosphatidylinositol 3-kinase（PI3K）, vascular endothelial growth factor（VEGF） and Caspase 3. Results With the increase of concentration of AA, the 24 h optical density（OD） value of SKOV3 and HO8910 cell lines increased significantly（P〈 0.05）. After 24 h, the proliferation activity of SKOV3 and HO8910 enhanced obviously in group 10 and 15μmol/L when compared with control subjects（P〈 0.05）, and m RNA and protein expression of PI3 K and VEGF upregulated significantly（P〈 0.05）, while, in contrast, m RNA and protein expression of Caspase 3 down-regulated in comparison to untreated control cells（P〈 0.05）. Conclusion Our results suggest that AA may promote the proliferation, invasion, metastasis and lamellipodia formation in ovarian cancer cell lines via PI3 K, VEGF and Caspase 3 pathways.
出处
《解放军医学院学报》
CAS
2016年第8期869-872,883,共5页
Academic Journal of Chinese PLA Medical School
基金
辽宁省科学技术计划项目(2014225005)~~
关键词
漆树酸
卵巢癌细胞
细胞迁移分析
anacardic acid
ovarian cancer cell
cell migration assay
