摘要
目的:考察三叶虫茶不同极性部位对溃疡性结肠炎(UC)模型大鼠的改善作用。方法:80%乙醇提取三叶虫茶得到总浸膏,用水分散后依次用三氯甲烷、乙酸乙酯进行萃取,挥干得相应部位浸膏。将60只大鼠随机分为正常组、模型组、阳性组(柳氮磺吡啶肠溶片,312.5 mg/kg)、三氯甲烷部位组(70 mg/kg)、乙酸乙酯部位组(35 mg/kg)和水部位组(320 mg/kg)。除正常组外,其余各组大鼠均采用2,4,6-三硝基苯磺酸(TNBS)法复制UC模型。24 h后,各给药组大鼠ig相应药物,正常组和模型组大鼠ig生理盐水,每天1次,连续14 d。观察大鼠结肠组织病理变化,对结肠黏膜损伤指数(CMDI)进行评分;测定血清中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果:与正常组比较,模型组大鼠发生明显UC样病变,CMDI评分升高;血清中SOD活性降低、MDA含量增加(P<0.01)。与模型组比较,各给药组大鼠病变程度减轻或好转,CMDI评分降低,其中乙酸乙酯部位组、阳性组差异有统计学意义(P<0.01);除三氯甲烷部位组外,其余各给药组大鼠血清中SOD活性均升高、MDA含量均减少,其中乙酸乙酯部位组SOD活性以及阳性组SOD活性、MDA含量差异有统计学意义(P<0.05或P<0.01)。结论:三叶虫茶乙酸乙酯部位可减轻结肠脂质过氧化反应,对TNBS致UC模型大鼠有明显的改善作用。
OBJECTIVE： To explore the improvement effect of different polar parts of Sanye Chongcha on models rat with ul- cerative colitis （UC）. METHODS： The total extract was obtained from Sanye Chongcha by extraction with 80% ethanol, and dis- persed in water, from which the extracts of corresponding parts were obtained after extraction successively with trichloromethane and ethyl acetate and drying. 60 rats were randomized into normal group, model group, positive group （sulfasalazine enteric-coated tablets, 312.5 mg/kg）, trichloromethane extraction part group （70 mg/kg）, ethyl acetate extraction part group （35 mg/kg） and wa- ter extraction part group （320 mg/kg）. The rats except for normal group received 2,4,6-trinitrobenzenesulfonic acid solution （TN- BS） to establish UC model. 24 h later, the rats in the drug administration groups were given corresponding drugs ig, while those in normal group and model group were given normal saline ig, once a day, for 14 consecutive days. For the rats, the pathological change of the colon tissue was observed, and the colon mucosa damage index （CMDI） was scored; the activity of superoxide dis- mutase （SOD） and the content of malondialdehyde （MDA） in serum were determined. RESULTS： Compared to normal group, the rats in model group had obvious UC-like lesion, higher CMDI, lower activity of SOD and higher content of MDA in serum （P〈 0.01）. Compared to model group, the rats in the drug administration groups had the lesion which became less serious or improved and had lower CMDI score, among which those of the ethyl acetate extraction part group and the positive group demonstrated a sta- tistically significant difference （P〈0.01） ; and the rats in the drug administration groups except for trichloromethane extraction part group had higher activity of SOD and lower content of MDA in serum, among which the activity of SOD of the ethyl acetate ex- traction part group and the activity of SOD and content of MDA of the positive group demonstrat
出处
《中国药房》
CAS
北大核心
2016年第25期3481-3484,共4页
China Pharmacy
基金
湖南省科技厅科研条件创新专项(No.2012TT2012)
湖南省教育厅重点项目(No.14A109)
湖南省2013年科技基本建设项目(No.湘发改高技〔2013〕1199号)
湖南省教学改革研究项目(No.2012-202)
湖南中医药大学青年教师基金项目(No.99820001134)
湖南中医药大学研究生科研创新项目(No.2015CX19)
湖南省研究生科研创新项目(No.CX2015B336)
关键词
三叶虫茶
醇提物
溃疡性结肠炎
超氧化物歧化酶
丙二醛
不同极性部位
大鼠
Sanye Chongcha
Alcohol extract
Ulcerativecolitis
Superoxide dismutase
Malondialdehyde
Different po-lar parts
Rats
