摘要
目的:探讨孕早期Ba P暴露对胚胎着床的影响,为进一步完善Ba P对雌性生殖功能的影响提供实验依据。方法:将60只孕鼠随机分为对照组和Ba P组,每组30只,每组再随机分成3小组,每组10只。从妊娠第1天对照组用玉米油、Ba P组用0.2 mg/(kg·d)剂量Ba P进行灌胃染毒,至妊娠的第4天(D4)、第5天(D5)、第6天(D6)处死并取材。计数小鼠胚胎着床点数目,ELISA方法检测小鼠血浆中雌激素和孕激素水平,荧光定量PCR、Western blot及免疫组织化学方法检测小鼠子宫内膜中雌激素受体-α(estrogen receptor-α,ERα)和孕激素受体(progesterone receptor,PR)水平。结果:比较对照组与Ba P组的胚胎着床数,Ba P组的着床点数在妊娠D5和D6均低于对照组,差异有统计学意义(P<0.05);Ba P组血浆雌激素水平和孕激素水平均高于对照组,差异均有统计学意义(P<0.05),不同染毒时间的雌激素水平与孕激素水平不同,由高到低依次为妊娠D6、D5、D4,各时间点的差异均有统计学意义(P<0.05);ERα的m RNA水平在Ba P组的表达高于对照组,差异有统计学意义(P<0.05),不同染毒时间ERα的m RNA水平不同,妊娠D6的表达高于妊娠D4和D5,差异有统计学意义(P<0.05),PR的m RNA水平在Ba P组的表达低于对照组,差异有统计学意义(P<0.05)。Western blot和免疫组化结果也表明苯并(a)芘暴露组小鼠子宫中ERα表达量升高,PR表达量下降。Western blot和免疫组化结果也表明Ba P组小鼠子宫中ERα表达量升高,PR表达量下降。结论:Ba P可能通过影响孕鼠雌孕激素水平及PR的表达模式,最终导致胚胎着床点数的下降。
Objective： To illuminate the effects of benzo （a） pyrene （BaP） on embryo implantation during the early pregnancy. Methods： Totally 60 pregnant mice were randomly divided into two groups. The control group was administered corn oil only, and the treated groups were exposed to BaP at 0.2 mg/kg body weight by oral gavage once a day. Pregnant mice were killed on days 4-6 by cervical dislocation, 10 mice each day. The uteri tissues were collected. The numbers of the implantation sites were calculated. The levels of estradiol（E2） and progesterone（P4） in plasma were determined by ELISA. qRT-PCR and Western blot were employed to detect the expression of the estrogen receptor-α （ERa） and the expression of progesterone receptor（PR）. Results：Compared with those of con- trol group, the numbers of embryo implantation sites were decreased in the BaP groups（P〈0.05）. The result of ELISA indicated that the levels of E2 and P4 in plasma were elevated in BaP treatment groups（P〈O.05）, qRT-PCR showed that the mRNA level of ERα was up-regulated （P〈O.05） and mRNA level of PR was down-regulated in the BaP treatment groups （P〈0.05）. Western blot and IHC showed that expression of ERα was up-regnlated while the expression of PR was down-regnlated after BaP exposure. Conclusion： BaP can cause the disorder of ERα and PR in the process of embryo implantation in mice,which will affect the expression pattern of ERα and PR and lead to the decrease in the number of embryo implantation.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2016年第7期675-679,共5页
Journal of Chongqing Medical University
基金
国家自然科学基金资助项目(编号:81202234
81573175)
重庆市科学技术委员会资助项目(编号:cstc2013jcyjA10046)
关键词
苯并(A)芘
雌孕激素
胚胎着床
小鼠
bcnzo （a）pyrene
estradiol and progesterone
em-bryo implantation
mice
