摘要
本文旨在研究解淀粉芽孢杆菌41B-1对花生白绢病的生物防治效果。采用对峙培养法,测定41B-1对白绢病菌的抑制效果;在温室盆栽条件下,用41B-1发酵菌液对花生进行灌根,然后挑战接种白绢病菌,检测对白绢病的防效。结果表明,41B-1能抑制白绢病菌的菌丝生长速率,破坏菌丝结构,减少菌核数量;41B-1能在培养基质和植株根系内部长期生存,并诱导植株产生防御反应,灌根处理植株对白绢病表现出明显的抗性,防效高达93.9%。因此,41B-1菌株可以作为防治花生白绢病的生防菌剂。
To determine effect of Bacillus amyloliquefaciens 41B- 1 in suppression of stem rot disease of groundnut plants caused by Sclerotium rolfsii,the inhibitory efficiency of 41B- 1 on hyphal growth of Sclerotium rolfsii on PDA were detected by coculture method. Under greenhouse conditions,we observed the resistant ability of groundnut plants treated with 41B- 1 after a challenging inoculation with Sclerotium rolfsii. Results showed that strain 41B- 1 significantly inhibited hyphal growth,destroyed hyphal structure and reduced the number of sclerotia. The results also showed that 41B- 1 strain had good colonization ability in the roots of plants,in addition,the colonization of Sclerotium rolfsii in the roots of plants was reduced and the disease index was significantly lower than that in control. 41B- 1 strain exhibited greater than 90% biocontrol efficacy against Sclerotium rolfsii in groundnut plants under greenhouse conditions. 41B- 1 strain provided a promising strategy to control stem rot disease of groundnut caused by the fungal pathogen Sclerotium rolfsii.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2016年第4期487-494,共8页
Chinese Journal of Oil Crop Sciences
基金
江苏省农业科技自主创新资金CX(14)2001
