摘要
研究克隆了斑马鱼(Danio rerio)abcc2基因序列,探讨了其在微囊藻毒素(MC-LR)解毒中的潜在功能。结果表明:斑马鱼abcc2具有同哺乳动物ABCC2相似的介导荧光底物MCB外排的转运活性,MC-LR处理可显著诱导其在斑马鱼幼体中的转录表达;过表达Abcc2蛋白能显著增强ZF4细胞和斑马鱼胚胎对MC-LR的耐受性;Abcc2作为MC-LR的主要耐受因子,在组织防御和有毒物质的排泄中起重要作用,但其解毒功能还不清楚。研究结果为进一步揭示鱼类抗MC-LR积累的分子机理及培育低MC-LR残留的养殖新品种提供理论基础。
Eutrophication in aquatic environment caused the release of microcystin-LR （MC-LR） into a water body during a period of cyanobacterial blooms, which pose serious threat to the fish survival and public health. In this study, we cloned the cDNA sequence of zebrafish （Danio rerio） abcc2 gene, and explored its role in MC-LR detoxification. Our results showed that zebrafish abcc2, similar to its mammalian counterparts, played roles in transport of its fluorescent substrate MCB, and that MC-LR treatment induced its transcriptional expression. Overexpression of Abcc2 significantly improved the survival rates of ZF4 cells and zebrafish embryos exposed to MC-LR. Zebrafish Abcc2 mediates MC-LR resistance and tissue defense and toxicants excretion, but it＇s role the detoxification of MC-LR remains unclear. These data reveal molecular mechanisms of fish in preventing accumulation of MC-LR and provide theoretical fundamentals for breeding cultured fish strains with MC-LR-resistant ability.
出处
《水生生物学报》
CAS
CSCD
北大核心
2016年第5期997-1002,共6页
Acta Hydrobiologica Sinica
基金
国家农产品质量安全风险评估项目子课题(GJFP201501004)
国家自然科学基金项目(31172395)资助~~
