摘要
目的了解中哈边境地区臀突客蚤(Xenopsylla minax)中立克次氏体携带情况。方法收集阿拉山口口岸臀突客蚤样本,PCR扩增立克次体17kDa基因片段,PCR产物测序并BLAST比对,利用Mega 6.0构建分子遗传进化树。结果42.86%(3/7)的臀突客蚤携带立克次体,17kDa基因遗传进化树显示与Candidatus Rickettsia senegalensis和Rickettsia bellii同源性最高。结论中哈边境口岸地区臀突客蚤携带立克次体核酸。
In order to investigate the infection state of Rickettsiafrom X.minaxat China-Kazakh border,the X.minax were collected,and 17 kDa genes of Rickettsia were tested by PCR,and the sequence was analyed by BLAST and phylogenetic allysied by Mage 6.0.Showed that the positive rate of 17 kDa gene of Rickettsia was 42.86%（3/7）at Alashankou Port.The phylogenetic tree of Rickettsia17 kDa gene showed that the uncultured Rickettsiasp.was the highest homology with Candidatus Rickettsia senegalensis and R.bellii.In conclusion the X.minaxinfected Rickettsiaat China-Kazakh border.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2016年第8期755-759,共5页
Chinese Journal of Zoonoses
基金
国家自然基金(No.81560338)
新疆维吾尔自治区自然科学基金(No.201442137-1)联合资助~~
关键词
立克次体
臀突客蚤
PCR
中哈边境
序列分析
Rickettsia
Xenopsylla minax
PCR
China-Kazakh border
sequence analysis
