摘要
采用浊度法对高效聚磷菌P_2的生长趋势进行了测定,通过单因素设计,探究了培养温度、初始pH值、培养时间及微量元素添加量对高效聚磷菌P_2除磷性能的影响,利用Plackett-Burman设计从影响除磷率的6个因素中筛选出3个主效因素,最后通过Box-Behnken设计对除磷条件进行优化,以期最大限度的提高聚磷菌P_2除磷效率。结果表明,高效聚磷菌P_2在24 h后生长趋势达到稳定,且持续时间较长;响应面法优化菌株P_2的除磷最优条件为初始化学需氧量(COD)494.5 mg/L、初始pH值7.4、接种量5%、初始磷含量50 mg/L、培养温度35℃、培养时间10.5 h,此条件下对磷酸盐的积累能力最强,对磷酸盐的去除率可达到92.51%。
The growth trend of the efficient phosphorus-accumulating bacterum P2 was researched by testing turbidity value. The effect of temperature, initial pH, culture time and trace elements addition on phosphate removal efficiency was researched by single factor experiment. Three main factors were selected from six influencing factors by the design of Plackett-Burman. Finally, the response surface methodology experiment was designed to investigate the optimal phosphorus removal conditions, to maximumly improve phosphorus removal efficiency of strain P2. The results showed that the growth trend of efficient phosphorus-accumulating bacterum P2 was stable after 24 h, and sustained for a long time. The response surface method- ology results showed that the optimal phosphorus removal condition by strain P2 was initial COD concentration 494.5 rag/L, initial pH 7.4, inoculum 5%, initial phosphorus content 50 mg/L, temperature 35 % and time 10.5 h. Under the condition, the accumulation ability of the phosphate was the strongest, and the removal efficiency of phosphate was 92.51%.
出处
《中国酿造》
CAS
北大核心
2016年第8期104-109,共6页
China Brewing
基金
酿酒及生物技术四川省重点实验室项目(NJ2014-05)
泸州老窖科研奖学金项目(15ljzk06)
四川省大学生创新基金项目(201510622061)
四川理工学院培育项目(2015PY02)
自贡市科技局(2015HX15)
关键词
聚磷菌
响应面法
除磷条件
phosphorus-accumulating bacterium
response surface methodology
phosphorus removal conditions
