摘要
目的初步探讨汉族上皮性卵巢癌(EOC)患者p53基因5-8外显子突变与"二元论"发病分型间的关系。方法选取2011年8月-2012年12月行手术并病理确诊的四川省汉族原发性EOC患者45例,利用DNA测序检测p53基因5-8外显子突变情况,并依照"二元论"将EOC患者分型,结合患者临床病理,分析各型EOC患者p53基因突变情况。结果Ⅱ型EOC患者p53基因突变频率及效率高于Ⅰ型EOC患者,差异有统计学意义(P<0.01);且发现175位密码子可能是Ⅱ型EOC的突变热点。在临床病理参数方面Ⅱ型EOC恶性程度及输卵管受累率高于Ⅰ型EOC,恶性程度高的临床病理特征患者中p53突变率显著升高。结论 p53基因突变在Ⅱ型EOC发生发展过程中可能发挥重要作用,其中175位密码子可能是Ⅱ型EOC的突变热点。
Objective To explore the relationship between p53 mutation in 5-8 exons and type of epithelial ovarian cancer （EOC） pathogenesis of Hart nationality women. Methods From August 2011 to December 2012, 45 patients with primary EOC （Han nationality women from Sichuan Province） diagnosed surgically and pathologically were selected. Using direct DNA sequencing, we analyzed the mutations of p53 in 5-8 exons of all cases, and the EOC patients were divided into two types according dualism and the pathogenesis results. The p53 mutation of the different types in EOC patients were analyzed. Results The frequency and efficiency of p53 mutation in type- II EOC patients were significantly higher than that in type I （P 〈 0.01）. And the codon 175 might be a mutational hotspot of type-]I EOC. The malignant degree and oviduct involved frequency of type- lI EOC were obviously higher than that of type-I EOC; p53 mutation frequency in high malignant patients increased significantly. Conclusions p53 mutation plays an important role in the development of type- II EOC. The codon 175 might be a mutational hotspot of type-II EOC.
出处
《华西医学》
CAS
2016年第8期1389-1393,共5页
West China Medical Journal
基金
国家自然科学基金(61505126)~~
