摘要
【目的】利用ISSR分子标记技术对选择的富士芽变优系和12个苹果品种为材料进行分子鉴定,构建指纹图谱,并且根据聚类分析的结果确定这13个苹果品种的亲缘关系。【方法】从100条ISSR引物中对供试样品进行PCR扩增筛选,采用NTSYSpc2.10t软件根据Nei’s遗传相似系数采用UPGMA法进行聚类,此分析亲缘关系,并采用POPGENE1.32软件计算多态性位点数、多态性点百分率,最终筛选出条带清晰、多态性较高、稳定且重复性好的引物。【结果】从100个ISSR引物中筛选出3个多态性高、重复性好的引物,分别为UBC846、UBC881和UBC899,共在57个位点扩增出条带,其中50个为多态性位点,平均多态性位点百分率为86.11%。利用3条多态性ISSR引物构建的数字化指纹能够有效地区分出所有供试材料,且建立了13个苹果品种的亲缘关系聚类图,这13个品种的相似系数的变化范围在0.67～0.82,表现出较高的遗传多样性。【结论】ISSR分子标记技术可以有效地用于苹果种质资源评价、指纹图谱的构建和苹果芽变品种的鉴定,为苹果的种质资源鉴定、遗传多样性检测和栽培育种提供了分子生物学依据。
【Objective】This project aims to do molecular identification of bud sprouts of the Fuji apples and 12 apple cultivars chosen and construct the fingerprint by using ISSR molecular markers technique,and according to the result of clustering analysis to determine the genetic relationships of the 13 apple cultivars.【Method】From 100 ISSR primers on the tested samples for PCR amplification and selection,using NTSYS-pc2. 10 t according to Nei＇s genetic similarity coefficient UPGMA clustering,in order to analyze the genetic relationship. The number of polymorphism and the percentage of polymorphic loci were calculated by POPGENE1. 32 and eventually were screened out with clear stripe,rich polymorphism,repeatability,and the varieties with high resolution.【Result】Four ISSR primer pairs with high polymorphism and good repeatability were selected from 100 ISSR primer pairs,and they were named as UBC846,UBC881 and UBC899. Four primer pairs produced 57 loci,which 50 were polymorphic. The percentage of polymorphic loci was 86. 11%. Three polymorphic ISSR primers used to construct the digital fingerprint could effectively distinguish all the materials,and establish the phylogenetic relationships of 13 apple varieties. The similarity coefficient of the 13 varieties ranged between 0. 67- 0. 82,showing a higher genetic diversity.【Conclusion】ISSR molecular marker technology can be effectively used to identify apple germplasm resources evaluation,fingerprint construction and bud mutation varieties identification and it is suitable for the germplasm identification and genetic relationship analysis of apple,thus providing a biological theoretical foundation for breeding and cultivation.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2016年第7期1230-1236,共7页
Xinjiang Agricultural Sciences
基金
国家林业公益性行业科研专项课题"新疆特色林果优良品种选育和推广"(201304701-1)
自治区特色林果发展专项林木遗传育种项目(2014年)~~
