摘要
目的探讨自然杀伤细胞(natural killer cell,NK)与早发型重度子痫前期发生、发展的相关关系。方法选取2012年1月至2014年12月在北京潞河医院定期孕检的重度子痫前期患者44例(早发型重度子痫前期20例,晚发型重度子痫前期24例)为研究对象。选取同时期正常无合并症的患者20例为对照组。采集母亲外周血2 m L,胎儿脐带血2 m L及蜕膜组织。采用流式细胞学方法检测外周血和脐带血中的CD3-CD16-CD56+的百分率;蜕膜组织应用免疫组织化学方法,计算CD3-CD16-CD56+的数量取均值,应用计算机图像分析法读取CD56+细胞平均灰度值。结果重度子痫前期组外周血、脐血、蜕膜组织中的NK细胞数量高于对照组,差异有统计学意义(均P<0.05);早发型重度子痫前期组脐血、蜕膜组织中NK细胞数量高于晚发型重度子痫前期组,差异有统计学意义(P<0.05)。重度子痫前期外周血、蜕膜组织中的NK细胞数量与病程进展呈负相关(r=-0.397,P=0.040;r=-0.391,P=0.044),与血压升高幅度呈正相关(r=-0.597,P=0.001;r=-0.416,P=0.031)。蜕膜组织中的NK细胞数量与确诊重度子痫前期的孕周呈负相关(r=-0.594,P=0.003)。结论重度子痫前期患者外周血、脐血、蜕膜中NK细胞数量增加,早发型重度子痫前期增加更为显著。外周血、蜕膜中NK细胞数量与早发型重度子痫前期的发生、发展相关。
Objective To study the correlation of natural killer cell（ NK） to early onset preeclampsia. Methods This research adopts the case-control study. 44 preeclampsia patients who were regularly checked up in Bei Jing LUHE hospital from Jan 2012 to Dec2014 entered the research. The group included 20 early onset preeclampsia patients and 24 late onset preeclampsia patients. The control group was normal patients at the same period. Collecting maternal peripheral blood umbilical blood and deciduas. We test percentage of NK cell in peripheral and umbilical blood by Flow cytometry. NK cells in deciduas were tested by immunohistochemistry. Results The courts of NK cells in maternal peripheral,umbilical blood and deciduas of early onset preeclampsia and late onset preeclampsia were all higher than those of the normal（ P〈0. 05）. It is significantly different. The counts of the NK cells in umbilical blood and deciduas were significantly higher than those of the late onset preeclampsia patients（ P〈0. 05）. The NK cell percentage in maternal peripheral blood in early onset preeclampsia patients were higher than in late onset preeclampsia patients. P〈0. 05. The courts of NK cells in maternal peripheral and deciduas was with negative correlation with course of preeclampsia.（ r =-0. 397,P = 0. 040; r =-0. 391,P = 0. 044）. It was positive correlation with the degree of elevation of pressure.（ r =-0. 597,P = 0. 001; r =-0. 416,P = 0. 031）. The courts of NK cells in deciduas was positive correlation with the time of diagnosis.（ r =- 0. 594,P = 0. 003）. Conclusion The counts of the NK cell in maternal peripheral blood,umbilical blood and deciduas in preeclampsia patients were significantly higher than those of the control. Early onset severe preeclampsia increased significantly. NK cell in maternal peripheral blood and deciduas probably play a role in early onset preeclampsia.
出处
《首都医科大学学报》
CAS
北大核心
2016年第4期538-542,共5页
Journal of Capital Medical University
