摘要
目的研究四川省小肠结肠炎耶尔森菌(Y.e)在常见宿主及患者中的分布特征。方法在不同季节采集不同地区腹泻患者或宿主动物进行常规分离鉴定。对分离菌株进行血清学和生物学分型,同时用PCR方法检测毒力基因。结果从1557份各类样品中分离出Y.e 47株,其中腹泻患者7株,宿主动物猪25株,青海田鼠10株,喜马拉雅旱獭5株;总分离率3.03%。血清型分布为O∶3血清型菌株27株,O∶9血清型2株,O∶5血清型6株,O∶8血清型2株,不能分型10株;毒力基因检测情况表明除青海田鼠分离的到4株O∶3血清型菌株外,其余O∶3、O∶9血清型菌株均具有致病性。结论四川省腹泻患者与常见宿主动物均存在小肠结肠炎耶尔森氏菌感染,感染率存在季节差异;首次证实在四川高原野生动物喜马拉雅旱獭中存在致病性Y.e,并证实宿主动物O∶3血清型中存在不产毒力基因的非致病性菌株。
Objective The distribution of Yersinia enterocolitica in patient and animal host in Sichuan Province was investigated. Methods Y. e were isolated from various kinds of samples in different seasons and different areas by conventional methods and the isolates were typed with serological methodsand biological typing assay. In addition,virulence genes were detected by PCR method. Results 47 strains of Y. e were isolated from 1 557 kinds of samples,including 7 strains from diarrhea patients,25 strains from the pigs,10 strains from Microtus fuscus,5 strains from Marmota himalayana. The isolation rate was 3. 03%. O∶ 3 serotype were 27 strains,O∶ 9serotypes were 2 strains,O∶ 5 serotype were 6 strains,O∶ 8 serotype were 2 strains,10 strains can not be typed.We have isolated 7 strains from patient,25 strains from pigs,10 strains from Microtus fuscus and 5 strains from Marmota himalayana. Detection of virulence genes show that except the 4 O∶ 3 strains from Microtus fuscus,the rest of the O∶ 3 and O∶ 9 strains are pathogenic. Conclusion The infection of Yersinia enterocolitica in occurs both in the patients and common host animals in Sichuan Province,detection rates of seasonal differences exist.It is the first time to prove that Y. e exists in wildlife in Sichuan, and confirme nonpathogenic strain in O ∶ 3serotype strains.
出处
《预防医学情报杂志》
CAS
2016年第9期895-898,共4页
Journal of Preventive Medicine Information
基金
国家科技重大专项(2012ZX10004-201)
