摘要
目的观察枳壳甘草汤对大鼠腰椎间盘突出模型移植髓核组织中PLA2的活性、白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)表达的影响,探讨枳壳甘草汤对减轻大鼠腰椎间盘突出模型炎症反应及延缓椎间盘退变的机制。方法将SD雄性大鼠90只,采用随机数字表法随机分为3组,每组30只,即模型组、中药组(枳壳甘草汤)、西药组(吲哚美辛)。于造模后7、14、28 d分别取出移植髓核,观察移植髓核组织内PLA2的活性值变化;采用免疫组化的方法观察IL-6和TNF-α的表达情况。结果 1)造模成功经大鼠灌胃后第7日,各实验组PLA2的活性达到最高峰经枳壳甘草汤干预7 d后PLA2活性值明显下降,但不如西药组明显。但在14、28 d后枳壳甘草汤组髓核组织中PLA2活性值与西药组差异不明显,中、西药物在降低PLA2活性值方面差异无统计学意义(P>0.05)。2)在造模28 d后,各组移植髓核组织内阳性细胞反应结果为相应部位呈棕黄色或棕褐色染色。其中模型组IL-6和TNF-α表达高于西药组和中药组(P<0.05或P<0.01),中、西药组比较差异无统计学意义(P>0.05)。结论 1)枳壳甘草汤能一定程度上降低突出髓核组织中PLA2活性值的作用,起到和抗炎镇痛类西药同样的效果。2)枳壳甘草汤能抑制移植髓核组织中阳性细胞IL-6和TNF-α的表达,抑制炎症介质的合成,对延缓椎间盘的退变起到一定的作用。
Objective：To observe the effects of Zhike Gancao decoction on PLA2 activity,IL-6 and TNF-α expression transplanted nucleus pulposus tissue of lumbar disc protrusion model and explore the mechanism on reducing inflammation and degeneration of lumbar disc protrusion model.Methods：90 SD male rats were randomly divided into for three groups with random number table method,with 30 rats in each,namely,the model group,TCM group（Zhike Gancao decoction）,and western medicine group（indomethacin）.On 7th,14 thand 28thday after modeling,the nucleus pulposus was removed and changes of PLA2 activity values were observed;the expression of IL-6 and TNF-α were observed with immunohistochemical method.Results：1）On seventh days after intragastric administration,the activity of PLA2 in each group reached the highest peak.On the seventh day,PLA2 activity of the TCM group dropped obviously,but not as obvious as the western medicine group.But on 14 thand 28thday,PLA2 activity of the nucleus pulposus tissue in Zhiqiao Gancao decoction group was not obvious different from of western medicine group;there was no difference in the decrease of PLA2 activity between Chinese medicine and the western medicine（P 0.05）.2）28th day after modeling,the reaction result of positive cells in the nucleus pulposus in each group was that the corresponding areas were yellow or brown stain.Among them,the expression of IL-6 and TNF-in model group was higher than that in western medicine group and TCM group（P 0.05 or P 0.01）,and there was no obvious difference between TCM group and the western medicine group（P 0.05）.Conclusion：1）Zhike Gancao decoction can reduce the role of PLA2 in the nucleus pulposus tissue to a certain extent,and has the same effect as anti-inflammation and analgesic effect;2）Zhike Gancao decoction can inhibit the expression of IL-6 and TNF-α in the positive cells of the nucleus pulposus,inhibit the synthesis of inflammatory mediators,and play a role in delaying the degeneration of intervertebral disc.
出处
《中国中医急症》
2016年第8期1488-1492,共5页
Journal of Emergency in Traditional Chinese Medicine
基金
苏州市科技局科研专项课题(SYSD2014183)
关键词
枳壳甘草汤
大鼠腰椎间盘突出模型
PLA2活性
IL-6/TNF-α的表达
椎间盘退变
Zhike Gancao decoction
Model of lumbar intervertebral disc herniation in rats
PLA2 activity
Expression of IL-6/TNF-α
Intervertebral disc degeneration
