摘要
目的：研制一种适合现场快速鉴定减肥类保健食品中酚酞的检测卡。方法：依据酚酞在碱性体系中反应形成粉红色醌式结构物原理,通过物理吸附将碱性体系中的有效组分附着到中性滤纸的材质上制备酚酞检测卡。酚酞检测卡与标准色阶图谱对比进行定性分析,与食品中危害因子及非法添加药物智能化综合检测仪配合;根据反射光谱法原理,将0.50 mg/g、1.00 mg/g、2.00 mg/g、3.00 mg/g和6.00 mg/g酚酞标准品与碱性体系反应生成醌式结构物浓度值内部拟合形成标准曲线,可定量分析确定待检品中酚酞的浓度。结果：酚酞检测卡在食品中危害因子及非法添加药物智能化综合检测仪检测以及同药典液相检测方法进行对比实验的结果符合率为90%~98%,证实酚酞检测卡具备较高的灵敏度。结论：研制的酚酞检测卡即可定性检测又可定量检测分析,适合各种快速检测减肥类保健食品酚酞含量的要求。
Objective： To develop a rapid detection card of Phenolphthalein illegal add chemicals in the health weight-reducing food suitable for rapid identification on site. Methods： On the basis of phenolphthalein pink complex principle in alkaline system reaction, the effective components of alkaline system attached to the neutral filter material on the preparation of phenolphthalein test card by physical adsorption. The qualitative analysis was analyzed between phenolphthalein test card and standard color order spectrum, which coordinated with harmful factors in food and illegal add drugs intelligent integrated detector. According to the principle of reflection spectroscopy, phenolphthalein standard reaction of 0.50mg/g, 1.00mg/g, 2.00mg/g, 3.00mg/g and 6.00mg/g and alkaline system generated inside the complex density form standard curve fitting, in order to analyze the concentration quantitatively to determine the quarantine of phenolphthalein. Results： Using phenolphthalein test card harmful factors in food drugs, illegal add intelligent integrated detector test results and comparative experiments with pharmacopoeia liquid phase detection method, the coincidence rate were from 90% to 98%, confirming phenolphthalein dipstick test card has higher sensitivity. Conclusion： Development of phenolphthalein inspection card can be used in qualitative test and quantitative analysis, suitable for all kinds of rapid detection of the requirements of the content of phenolphthalein health weight-reducing food.
出处
《中国医学装备》
2016年第8期11-17,共7页
China Medical Equipment
关键词
检测卡
酚酞
保健食品
试纸
快速
减肥类
Test card
Phenolphthalein
Health food
Test paper
Rapid
Slim chemical composition
