摘要
目的探讨7-硝基吲唑(7-NI)对创伤性颅脑损伤(TBI)大鼠血清神经元特异性烯醇化酶(NSE)和星形胶质源性蛋白(S100β)水平的影响。方法健康成年SD大鼠160只随机均分为生理盐水处理组和7-NI处理组;每组又均分为假手术和TBI后6、12和24h四小组。建立大鼠可控性皮质损伤模型。检测指标:血清NSE和S100β蛋白浓度,脑组织一氧化氮(NO)含量和神经元型一氧化氮合酶(nNOS)活性,脑组织含水量,血-脑脊液屏障(BBB)通透性,脑组织丙二醛(MDA)和总超氧化物歧化酶(T-SOD)含量。HE染色评价脑损伤严重程度。结果随TBI后时间推移,两组大鼠TBI后血清NSE和S100β蛋白浓度、脑组织NO含量和nNOS活性、脑组织含水量、BBB通透性和MDA含量均较假手术组增高(P<0.05),脑组织T-SOD含量下降(P<0.05),脑损伤逐步加重。在TBI后6h和12h,7-NI处理组血清NSE和S100β蛋白浓度、脑组织NO含量、脑组织nNOS、活性脑组织含水量、BBB通透性和MDA含量均低于生理盐水处理组(P<0.05);在伤后6h和12h,7-NI处理组脑组织T-SOD含量高于生理盐水处理组(P<0.05);在TBI后12h内,7-NI处理组脑损伤程度低于生理盐水处理组(P<0.05)。结论 TBI后早期应用7-NI可以减轻脑组织水肿,降低BBB通透性,减少脑组织氧化应激。血清NSE和S100β蛋白浓度可以反映7-NI的治疗效果。
Objective To investigate the role of 7-nitroindazole （7-NI） in regulating serum levels of neuron specific enolase（NSE） and $100t3 in rat model with traumatic brain injury（TBI）. Methods A total of 160 healthy adult male SD rats was equally randomized into two groups of A （ treated with normal saline） and B（treated with 7-NI）, which were equally subgrouped as four groups of sham operation, and 6 hours, 12 hours and 24 hours after TBI. The rat models with controlled cortical strike were established. The indicators measured included serum NSE and S100β, the contents of nitric oxide（NO） and the activity of neuronal nitric oxide synthase（nNOS） in brain tissues, brain water, the permeability of blood cerebrospinal fluid barrier, the contents of malondialdehyde （MDA） and total superoxide dismutase（T-SOD） in brain tissues. Pathological changes of brain tissues were detected by HE staining. Results As time went on, serum levels of NSE and S100β, the contents of NO and the activity of nNOS in brain tissues, brain water, the permeability of blood-cerebrospinal fluid barrier, and the content of MDA were increased（P〈0. 05）, T-SOD brain tissues decreased（P〈0. 05） and brain damage aggravated after TBI. At 6 and 12 hours after TBI, 7-NI treated groups showed decreased serum NSE and S100β levels, the contents of NO, the activity of nNOS, the contents of water in the brain, the permeability of blood-cereb~＇ospinal fluid barrier and the content of MDA compared to saline-treated groups（P〈00. 05）. Compared to saline-treated groups, T-SOD in brain tissues at 6 and 12 hours after TBI was higher（P〈0. 05） ,and the severity of brain injury was lower in 7-NI treated-groups（P〈0. 05） . Conclusion In the rats with controlled cortical strike, early using 7-NI can significantly alleviate brain edema, reduce the permeability of blood cerebrospinal fluid barrier, and attenuate the oxidative stress of brain tissues. Serum levels of NSE and S100β may reflect the outcomes of 7-NI
出处
《江苏医药》
CAS
2016年第15期1644-1647,1651,F0003,共6页
Jiangsu Medical Journal
关键词
颅脑损伤
7-硝基吲唑
氧化应激
Traumatic brain injury
7-Nitroindazole
Oxidative stress
