摘要
目的探讨不同胎龄早产儿脐带血中内皮祖细胞（EPC）水平及迁移能力的差异。方法选择2014年10月至2015年7月,于四川大学华西第二医院、四川省医学科学院·四川省人民医院、成都市第一人民医院收集的47份新生儿的脐带血为研究对象。根据收集脐带血的新生儿胎龄,将其分为极早产儿组（胎龄为28-32孕周,n=12）,早产儿组（胎龄为32-36孕周,n=20）,以及足月儿组（胎龄〉37孕周,n=15）。将3组新生儿脐带血于诱导分化前,采用流式细胞仪检测脐带血中EPC水平;于诱导分化后,采用荧光探针标记的乙酰化低密度脂蛋白（Dil-Ac-LDL）与异硫氰酸荧光素标记的荆豆凝集素（FITC-UEA-1）对单个核细胞进行孵育、染色,荧光显微镜下观察3组单个核细胞染色情况,判断EPC是否诱导分化成功。采用Transwell迁移实验,检测3组新生儿脐带血EPC迁移能力。统计学比较3组新生儿脐带血诱导分化前EPC水平,诱导分化后EPC迁移数量。本研究遵循的程序符合收集脐带血的上述3家医院伦理委员会所制定的伦理学标准,得到批准,脐带血获取前,均征得产妇的知情同意,并与之签署临床研究书知情同意书。3组收集脐带血的新生儿性别构成比、分娩方式及出生时Apgar评分等基本资料比较,差异均无统计学意义（P〉0.05）。结果 1流式细胞仪检测结果显示,脐带血诱导分化前,3组新生儿脐带血中,EPC水平比较,差异无统计学意义（F=0.302,P=0.654）。2Dil-Ac-LDL、FITC-UEA-1双荧光染色结果显示,3组新生儿脐带血中EPC均诱导分化成功,并且随着胎龄增加,单个核细胞诱导分化为EPC的数量逐渐增多。3Transwell迁移实验结果显示,极早产儿组、早产儿与足月儿组新生儿脐带血中EPC迁移数量分别为（13.8±21.7）个/视野、（18.8±12.4）个/视野与（39.9±23.8）个/视野,3组比较,差异有统计学意义（F=3.826,P=0.027）。其中,极早产儿组与�
Objective To investigate the differences of the number and migration ability of endothelial progenitor cell（EPC）isolated from umbilical cord blood among preterm infants with different gestational ages.Methods From October 2014 to July 2015,a total of 47 cases of umbilical cord blood of neonates in West China Second University Hospital,Sichuan University,Sichuan Academy of Medical Sciences Sichuan Provincial People′s Hospital,and Chengdu First People′s Hospital were chosen into this study.According to the gestational age of newborns whose umbilical cord blood was collected,they were divided into very preterm group（the gestational ages were 28to32 weeks,n=12）,preterm group（the gestational ages were 32 to 36weeks,n=20）,and full-term group（the gestational ages were 37 weeks,n=15）.Before the induced differentiation of umbilical cord blood in the three groups,flow cytometry was used to detect the level of EPC.After the induced differentiation of umbilical cord blood in the three groups,the mononuclear cells were incubated and stained by fluorescent probes labeled Dil-acetylated low-density lipoprotein（Dil-Ac-LDL）and fluorescein isothiocyanate-labeled ulex europaeus agglutinin-1（FITC-UEA-1）.The stainings of mononuclear cells in the three groups were observed under fluorescence microscope to detect whether the EPC was successfully induced or not.Transwell migration assay was used to detect the migration ability of EPC in the three groups.The levels of EPC before the induced differentiation of umbilical cord blood in three groups and the migration numbers of EPC after the induced differentiation of umbilical cord blood in three groups were compared by statistical methods.The study protocol was approved by the Ethical Review Boards of three hospitals from which the umbilical cord blood was collected.Informed consent was obtained from each pregnant woman before obtaining umbilical cord blood.There were no significant differences in gender ratio,delivery method,Apgar scores and other basic data am
出处
《中华妇幼临床医学杂志（电子版）》
CAS
2016年第4期425-431,共7页
Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)
基金
四川省科技厅应用基础研究项目(2011JY0085)~~
关键词
内皮祖细胞
脐带血
新生儿
诱导
迁移能力
Endothelial progenitor cells
Umbilical cord blood
Infant
newborn
Induction
Migration ability
