摘要
【目的】研究普氏蹄蝠(Hipposideros pratti)胃肠道菌群多样性及致病菌的种类。【方法】采用Mi Seq高通量测序技术,通过对16S r RNA基因V1-V2区基因进行测序,研究普氏蹄蝠胃肠道细菌的群落组成。应用MG-RAST V3.3.6分析和统计样品序列和操作分类单元(OTU)数目,分析胃肠道菌群物种丰度,并进行聚类分析。【结果】从普氏蹄蝠胃和肠道中分别获得144 998条和275 274条原始序列以及48 332条和91 758条有效序列,分属于894个和756个操作分类单元。胃中菌群丰度指数Chao指数(1 490)和ACE指数(2 221)分别低于肠道菌群的Chao指数(2 051)和ACE指数(3 556);Shannon多样性指数(2.405)低于肠道(2.407);Simpson多样性指数(0.168)高于肠道(0.151)。系统进化分析表明胃肠中的细菌主要分布在6个门,均以变形菌门(Proteobacteria)(胃中占56.4%,肠中占46.0%)和厚壁菌门(Firmicutes)(胃中占40.7%,肠中占49.2%)为优势菌门。胃肠道中丰度在0.1%以上的属有24个,胃中优势类群为乳球菌属(Lactococcus)和哈夫尼菌属(Hafnia),分别占整个菌群的26.1%和21.0%;肠道中优势类群为肠球菌属(Enterococcus)和沙门氏菌属(Salmonella),分别占整个菌群的15.2%和12.7%。普氏蹄蝠胃肠道中的优势菌群均为人类的致病菌或者条件致病菌。【结论】普氏蹄蝠携带有大量人类致病菌。因此,应注意防止向人类传播。
[Objective] We studied the bacterial diversity and pathogenicity in the gastrointestinal tract of Hipposiderospratti. [Methods] The 16S rRNA gene V1-V2 genes were sequenced by using MiSeq high-throughput sequencing to study the bacterial community in stomach and intestine of H. pratti. MG-RAST V3.3.6 was used to evaluate the abundance, diversity and operational taxonomic units （OTUs） of the bacteria. [Results] In total 144 998 and 275 274 original sequences, and 48 332 and 91 758 high quality reads were obtained from the stomach and intestine ofH. pratti. They belonged to894 and 756 OTUs. The bacterial abundance indices （Chao, 1 490; ACE, 2 221） and Shannon diversity index （2.405） in the stomach were lower than those of intestinal tract （Chao, 2 051; ACE, 3 556; Shannon, 2.407）. On the contrary, Simpson diversity index （0.168） in the stomach was lower than that of intestinal tract （0.151）. Phylogenetic analysis results show that the gastrointestinal bacteria were mainly distributed in 6 Phyla, dominated by Proteobacteria （56.4% in stomach and 46.0% in intestines） and Firmicutes （40.7% in stomach and 49.2% in intestines）. There were 24 genera above 0.1% in stomach and intestine of H. pratti. Lactococcus and Hafnia were the 2 dominant bacterial genera in stomach accounting for 26.1% and 21.0% respectively. Enterococcus and Salmonella were 2 dominant genera in intestine accounting for 15.2% and 12.7%, respectively. Unfortunately, these dominant bacteria were human pathogenic bacteria or opportunistic pathogen. [Conclusion] H. pratti carried a large number of human pathogens and thus should be paid more attentions to prevent transmission of diseases from the bats to human.
出处
《微生物学通报》
CAS
CSCD
北大核心
2016年第8期1739-1745,共7页
Microbiology China
基金
国家自然科学基金项目(No.31172056)~~
关键词
普氏蹄蝠
胃肠道
菌群
多样性
Hipposideros pratti
Gastrointestinal tract
Bacterial community
Diversity
