Avoiding experimental bias by systematic antibody validation
Avoiding experimental bias by systematic antibody validation
摘要
Antibodies are valued reagents in most modern laboratories and indispensable for many experiments.They can be utilized in a wide range of experimental setups designed to elucidate various scientific questions.In our laboratory,antibodies are used to investigate protein localization,quantity and phosphorylation state,
Antibodies are valued reagents in most modern laboratories and indispensable for many experiments.They can be utilized in a wide range of experimental setups designed to elucidate various scientific questions.In our laboratory,antibodies are used to investigate protein localization,quantity and phosphorylation state,
参考文献8
-
1Arnett MG, Ryals JM, Wright DE (2007) Pro-NGF, sortilin, and p75NTR: potential mediators of injury-induced apoptosis in the mouse dorsal root ganglion. Brain Res 1183:3242. 被引量:1
-
2Baker M (2015) Reproducibility crisis: Blame it on the antibodies. Nature 521:274-276. 被引量:1
-
3Bordeaux J, Welsh A, Agarwal S, Killiam E, Baquero M, Hanna J, Anagnostou V, Rimm D (2010) Antibody validation. Biotechniques 48:197- 209. 被引量:1
-
4Gurgor P, Pallesen EF, Johnsen L, Ulrichsen M, de Jong IE, Vaegter CB (2016) Neuronal death in the dorsal root ganglion after sciatic nerve injury does not depend on sortilin. Neuroscience 319:1-8. 被引量:1
-
5Kobayashi K, Fukuoka T, Yamanaka H, Dai Y, Obata K, lbkunaga A, Noguchi K (2005) Differential expression patterns of mRNAs for P2X receptor subunits in neurochemically characterized dorsal root ganglion neurons in the rat. J Comp Neurol 481:377-390. 被引量:1
-
6Prassas I, Brinc D, Farkona S, Leung F, Dimitromanolakis A, Chrystoja CC, Brand R, Kulasingam V, Blasutig IM, Diamandis EP (2014) False biomarker discovery due to reactivity of a commercial ELISA for CUZD1 with cancer antigen CA125. Clin Chem 60:381-388. 被引量:1
-
7Rimm D (2010) Use and Abuse of Antibodies in the Lab and in the Clinic. 被引量:1
-
8Zhang XF, Han P, Faltynek CR, larvis MF, Shieh CC (2005) Functional expression of P2X7 receptors in non-neuronal cells of rat dorsal root ganglia. Brain Res 1052:63-70. 被引量:1
-
1陈明元.旋光法测定蔗糖转化反应的速率常数实验的一种偏差与处理[J].贵州教育学院学报,2002,13(2):55-57.
-
2肖永厚,陶伟川,王仰东,刘苏.水在13X/CaCl_2复合吸附剂上的吸附平衡和扩散[J].天然气化工—C1化学与化工,2010,35(2):28-31. 被引量:1
-
3刘加威,李京伟,白枭龙,班伯源,孙继飞,陈健.石英砂ICP-OES测试溶样方法对比研究:酸溶法和碱熔法[J].化学工程师,2017,31(4):21-24. 被引量:4
-
4窦红顺.水产品组胺检测方法的改进[J].福建轻纺,2013(6):37-42. 被引量:6