摘要
本试验旨在研究低蛋白质饲粮添加α-酮戊二酸（α-ketoglutarate,AKG）对生长猪氮以及钙、磷代谢的影响。试验选用初始体重为（34.98±2.18）kg的健康三元杂交（杜×长×大）猪18头,按体重相近、性别比例相同原则随机分为3组,每组6个重复,每个重复1头。各组AKG的添加水平分别为0（对照组）、1%和2%。先进行为期14 d的饲养试验,观察生长性能;然后进行消化代谢试验,试验期为7 d,前5 d为预试期,后2 d为粪尿收集期（全收粪法）。结果表明：1）1%AKG添加组有改善35～45 kg生长猪生长性能的趋势,具体表现为提高平均日增重（P=0.194 2）和平均日采食量（P=0.258 3）,降低料重比（P=0.419 7）。2）随着AKG添加水平提高,尿氮含量有下降趋势（P=0.143 2）,粪氮、总氮含量及总氮排放率极显著降低（P〈0.01）,氮表观消化率和蛋白质净利用率极显著提高（P〈0.01）。与对照组相比,1%与2%AKG添加组尿氮含量分别降低了13.31%和41.88%,粪氮含量分别降低了18.73%和54.69%,总氮排放率分别降低了20.57%和50.00%,氮表观消化率分别提高了2.60%和6.32%,蛋白质净利用率分别提高了2.68%和6.51%。3）2%AKG添加组钙食入量、粪钙和粪磷含量显著低于对照组（P〈0.05）,而钙表观消化率和磷表观消化率却显著高于对照组（P〈0.05）;尿钙和尿磷含量有低于其他各组的趋势,但各组间差异不显著（P〉0.05）。由此可见,在35～45 kg的生长猪饲粮中添加1%～2%AKG可有效降低机体氮、钙、磷排放,提高氮及钙、磷的利用率和日增重。
This experiment was conducted to investigate the effects of α-ketoglutarate（ AKG） supplementation in low protein diet on nitrogen and calcium（ Ca） phosphorus（ P） metabolism of grow ing pigs. Eighteen healthy crossbred（ Duroc×Landrace×Largew hite） pigs with an average body weight of（ 34.98 ± 2.18） kg were randomly allocated to 3 groups evenly with 6 replicates in each group and 1 pig in each replicate. AKG supplemental level was 0（ control group）,1% and 2%,respectively. All pigs were firstly fed for 14 d,and growth performance was measured,follow ed by digestion and metabolism test. The experiment lasted for 7 d,the former 5 d was pre-trail period,and the latter 2 d was excreta collection period by means of collection full excrements analysis method. The results show ed as follow s： 1） growth performance of grow ing pigs with the weight of 35 to 45 kg tended to improve in 1% AKG supplementation group,embodied as increasing the average daily gain（ P = 0.194 2） and the average daily feed intake（ P = 0. 258 3）,while decreasing the feed to gain ratio（ P = 0.419 7）. 2） With the increase of dietary AKG supplemental levels,the urine nitrogen content tended to decrease（ P = 0.143 2）,the contents of fecal nitrogen and total nitrogen as well as total nitrogen emission rate were extremely significantly decreased（ P〈0.01）,while the nitrogen apparent digestibility and net protein utilization were extremely significantly increased（ P〈0.01）. Compared to the control group,urine nitrogen content was decreased by 13.31% and 41.88%,fecal nitrogen content was decreased by 18.73% and 54.69%,total nitrogen emission rate was decreased by 20.57% and 50.00%,nitrogen apparent digestibility was increased by 2.60% and 6. 32%,and net protein utilization was increased by 2. 68% and 6. 51% in 1% and 2% AKG supplementation groups,respectively. 3） Compared to the control group,the 2% AKG supplementation group significantly decreased the Ca intake,contents of fecal Ca and P（ P〈0.0
出处
《动物营养学报》
CAS
CSCD
北大核心
2016年第7期2154-2161,共8页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家973项目专题(2013CB127306)
国家自然科学基金面上项目(31472107)
湖南省“湖湘青年科技创新人才”项目(2015RS4053)
中科院“百人计划”项目
关键词
Α-酮戊二酸
生长猪
氮代谢
钙磷代谢
α-ketoglutarate
growing pigs
nitrogen metabolism
Ca ＆ P metabolism
