摘要
目的探讨体外受精-胚胎移植(IVF-ET)后妊娠早期合并宫腔积血(IUH)的女性外周血氧化应激水平与正常妊娠时有无差异。方法选取经IVF-ET助孕获宫内妊娠合并子宫腔积血者121例为宫腔积血组(IUH组),匹配孕囊数及周期类型,选取同期经IVF-ET助孕获宫内妊娠且无子宫腔积血者121例为对照组(N-IUH组)。采用ELISA法检测受试者外周血清中活性氧(ROS)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)及丙二醛(MDA)水平。结果IUH组与N-IUH组各临床特征间差异无统计学意义。IUH组ROS水平高于N-IUH组,差异有统计学意义(P<0.05)。IUH组MDA水平高于N-IUH组,差异有统计学意义(P<0.05)。IUH组与IUH组SOD及GPx水平的差异无统计学意义。结论经IVF-ET后宫内早孕合并IUH的女性体内ROS和MDA水平高于N-IUH时,提示体内存在较高的氧化状态,IUH与氧化应激间可能存在相关性。
Objective To investigate oxidative stress level in the in vitro fertilization （IVF）-pregnancies diagnosed with intrauterine hematoma （IUH）. Methods A matched case-control study was performed on 242 women including 121 women with intrauterine hematomas and 121 controls without intrauterine hematomas. ELISA was used to measure the marernal serum reactive oxygen species （ROS）, superoxide dismutase （SOD）, glutathione peroxidase （GPx） and malondialdehyde （MDA） levels in cases and controls. Results There was no difference in maternal clinical characteristics between the eases and controls. Elevated maternal serum ROS level was found to be significantly much higher in pregnancies with IUH, regardless of singleton or twin pregnancies. And so was MDA. But no significant differences were found in the SOD and GPx levels between the cases and controls. Conclusion IVF- pregnancies with intrauterine hematomas during the first trimester show changes in oxidative stress level, which suggests a possible association with intrauterine hematoma and oxidative stress.
出处
《安徽医科大学学报》
CAS
北大核心
2016年第8期1164-1167,共4页
Acta Universitatis Medicinalis Anhui
基金
安徽省高校省级自然科学研究项目(编号:KJ2012A146)
关键词
氧化应激
活性氧
体外受精-胚胎移植
宫腔积血
oxidative stress
reactive oxygen species
in vitro fertilization and embryo transfer
intrauterine hematoma
