摘要
目的研究T细胞因子4(TCF4)在胃癌细胞中的表达以及对胃癌细胞增殖及衰老、凋亡中的作用。方法培养人胃癌细胞株MGC803、SGC7901、HSC44-PE、44As3、MKN45、NCI-N87以及人永生化胃黏膜上皮细胞株GES-1,使用Western blot法比较TCF4在各细胞之间的表达差异。选择其中TCF4表达高与低的细胞株,分别通过稳定转染TCF4表达质粒及dominant negative TCF4(dn TCF4)的方法上调与下调细胞TCF4表达,采用体外细胞增殖实验及实时无标记细胞检测分析(RTCA)等技术检测TCF4对胃癌细胞增殖与凋亡的影响。建立胃癌细胞裸鼠移植瘤模型,观察TCF4蛋白对胃癌移植瘤生长的影响。结果 TCF4在胃癌细胞中的表达高于对照GES-1细胞。胃癌细胞株按TCF4的表达量从高到低排列依次为NCI-N87、44As3、HSC44-PE、MGC803、MKN45、SGC7901。TCF4的表达与胃癌细胞的增殖能力呈正相关性,而与胃癌细胞的衰老、凋亡呈负相关性。结论 TCF4在胃癌细胞中呈高表达,其能促进胃癌细胞的增殖、抑制胃癌细胞的衰老及凋亡。
Objective To research the expression of T cell factor 4 （TCF4） and the function of proliferation, senescence and apoptosis in gastric cancer cells. Methods Human gastric cancer cell lines MGC803, SGC7901 , HSC44-PE, 44As3, MKN45, NCI-N87 and immortalized human gastric epithelial cell line GES-I were cultured and the differential expressions of TCF4 among them were detected by Western blot analysis, then TCF4 expression and dominant negative TCF4 plasmid were transfected into the lowest and highest one respectively. The influence of TCF4 in vitro cells was tested by cell proliferation assay and RTCA. Meanwhile, the model of nude mice bearing tumor was established to detect the effect in vivo. Results The expression of TCF4 in gastric cancer cells was higher than the control GES-1, the highest one was NCI-N87, followed by 44As3, HSC44-PE, MGC803, MKN45 and SGC7901. TCF4 was positively correlated with proliferation, and negatively associated with senescence, apoptosis in gastric cancer cells. Conclusion TCF4 is overexpressed in gastric cancer cells. It could promote the proliferation, inhibit the senescence and apoptosis of gastric cancer cells.
出处
《安徽医科大学学报》
CAS
北大核心
2016年第8期1092-1096,共5页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81071808)
安徽省自然科学基金(编号:1408085MH167)
安徽省科技计划项目(编号:1301042094)
关键词
胃癌
TCF4
增殖
凋亡
gastric cancer
TCF4
proliferation
apoptosis
