摘要
以紫芝(Ganoderma sinense)的担孢子萌发获得的单核菌株为试验材料,采用三轮杂交法、OWE-SOJ技术及核迁移技术对其担孢子单核体交配型因子进行准确测定。结果表明,紫芝为四级性异宗结合菌类,其原生质体单核化菌株交配型分离比例符合1:1的预期,不同交配型担孢子的单核体符合1:1:1:1的自由分离规律,采用OWE-SOJ与核迁移试验相互印证,可以对紫芝担孢子交配型进行准确测定。
Four mating types of basidispores from Ganderma sinense were determined using three-cycle mating system, OWE- SOJ technique and nuclear migration test. Test results showed that G. sinense was a kind of basidiomycete with tetrapolarity heterothallism. The different mating types of protoplasted monokaryon and basidispores were present in a 1：1 and 1：1：1：1 ratio. The nuclear migration test is consist with that of OWE-SOJ, and they could establish corroboration for each other. Therefore it was feasible to apply this method to determine the mating type of basidispores accurately.
出处
《中国食用菌》
2016年第4期24-27,共4页
Edible Fungi of China
基金
福建省种业创新与产业化工程专项项目(2014S1477-21)
福建省食用菌产业重大农技推广服务试点项目(KNJ153013)
关键词
紫芝
交配型
四级性
核迁移
OWE-SOJ
Ganoderma sinense
mating type
tetrapolarity
nuclear migration
OWE-SOJ
