摘要
目的:观察大鼠实验性牙髓炎过程三叉神经节中CX3CL1与CX3CR1的动态表达,探讨三叉神经系统参与牙髓炎病理改变的信号调节作用。方法:建立大鼠牙髓炎动物模型,应用免疫荧光染色方法检测三叉神经节中CX3CL1与CX3CR1的表达变化。结果:免疫荧光染色结果显示,CX3CL1免疫阳性卫星胶质细胞围绕在神经元周围,形成典型的环状结构,而CX3CR1主要表达在神经元细胞体上。统计学分析显示,CFA致炎后2周,CX3CL1阳性细胞率达到峰值,明显高于正常对照组;CFA致炎后24h及72h,CX3CR1阳性细胞率与正常对照组相比明显增高。结论:三叉神经系统通过调节CX3CL1与CX3CR1的表达参与牙髓炎症及痛觉过敏的发生发展。
Objective：To investigate thedynamic expressionofCX3CL1 and CX3CR1in the trigeminal ganglions（TGs）and furthermore toelucidate the potential roles ofCX3CL1 and CX3CR1in the trigeminal nervous system that involved in the development of the inflammatory response and hyperalgesia induced by pulpitis.Methods：An animal-model of pulpitiswas established.The dynamic expression ofCX3CL1 and CX3CR1inTGswasobserved byimmunofluorescencetechnique.Results：CX3CL1and CX3CR1-positive cells were found in boththe experimental and control TGs by immunofluorescentstaining.The expression of CX3CL1 profiles were presented typical morphology of circles surroundingthe neurons,while CX3CR1 wereexpressed inTG neurons.Statistical analysis revealed that the numbers ofCX3CL1 and CX3CR1-positive cells were increased induced byCFA-inflammation.The rate of CX3CL1-positive cellsreached the peak at 2weeksand decreased to normal level at 6weeks.The rate of CX3CR1-positive cells was significantly higherthanthat ofthe control group at 24 hours and 72 hoursafter CFA-inflammation.Conclusion：The expression of CX3CL1 and CX3CR1may participate in the inflammatory response of pulpitis and hyperalgesia intrigeminal nervous system.
出处
《口腔医学研究》
CAS
CSCD
北大核心
2016年第7期685-688,共4页
Journal of Oral Science Research
基金
辽宁省自然科学基金项目(2013023051)
教育部留学回国人员科研启动基金资助项目
