摘要
目的：建立同时测定野老鹳草中没食子酸、原儿茶酸、儿茶素、柯里拉京、短叶苏木酚含量的方法。方法：采用高效液相色谱法。色谱柱为Wonda Sil C18WR,流动相为乙腈-0.1%磷酸溶液（梯度洗脱）,流速为1.0 ml/min,检测波长为280 nm,柱温为40℃,进样量为20μl。结果：没食子酸、原儿茶酸、儿茶素、柯里拉京、短叶苏木酚的检测质量浓度线性范围分别为0.02～20.02、0.01～20.10、0.02～19.78、0.02～20.02、0.02～20.10（r=0.999 9、0.999 7、0.999 6、0.999 9、0.999 5）;精密度、稳定性、重复性试验的RSD〈3.0%;加样回收率分别为95.1%～100.6%（RSD=2.20%,n=6）、95.8%～100.6%（RSD=1.74%,n=6）、95.1%～101.9%（RSD=2.71%,n=6）、97.7%～103.1%（RSD=2.04%,n=6）、95.3%～99.0%（RSD=1.46%,n=6）。结论：该方法操作简便、结果准确、重复性好,可用于同时测定野老鹳草中没食子酸、原儿茶酸、儿茶素、柯里拉京、短叶苏木酚的含量。
OBJECTIVE：To establish a method for simultaneous determination of gallic acid,protocatechuic acid,catechuic acid,corilagin and brevifolincarboxylic acid in Geranium carolinianum. METHODS：HPLC was performed on the column of Wonda Sil C18 WR with mobile phase of acetonitrile-0.1% Phosphoric acid（gradient elution）at a flow rate of 1.0 ml/min,the detection wavelength was 280 nm,the column temperature was 40 ℃,and the volume injection was 20 μl. RESULTS：The linear range was0.02-20.02 for gallic acid（r=0.999 9）,0.01-20.10 for protocatechuic acid（r=0.999 7）,0.02-19.78 for catechuic acid（r=0.999 6）,0.02-20.02 for corilagin（r=0.999 9）and 0.02-20.10 for brevifolincarboxylic acid（r=0.999 5）;RSDs of precision,stability and reproducibility tests were lower than〈 3.0%;recoveries were 95.1%-100.6%（RSD=2.20%,n=6）,95.8%-100.6%（RSD=1.74%,n=6）,95.1%-101.9%（RSD=2.71%,n=6）,97.7%-103.1%（RSD=2.04%,n=6）and 95.3%-99.0%（RSD=1.46%,n=6）. CONCLUSIONS：The method is simple,accurate and reproducible,and can be used for the contents determination of gallic acid,protocatechuic acid,catechuic acid,corilagin and brevifolincarboxylic acid in G. carolinianum.
出处
《中国药房》
CAS
北大核心
2016年第21期2972-2975,共4页
China Pharmacy
基金
广东省中医药局项目(No.20141199)
