摘要
建立樟芝粗多糖的含量测定方法。以葡萄糖为对照品,采用3,5-二硝基水杨酸比色法,测定樟芝中还原糖和总糖的含量,并计算出总多糖的含量。研究结果表明多糖含量在0.25 mg/m L^1.00 mg/m L范围内标准曲线具有良好的线性关系,测得回归方程y=0.597 9x-0.037 8(R^2=0.999 6)。还原糖和总糖的平均加样回收率分别为101.65%(RSD=1.89%)和100.92%(RSD=2.45%)。樟芝中总多糖含量为129.5 mg/g^148.1 mg/g。本方法灵敏、稳定、重复性好,有助于樟芝质量控制方法的研究。
A method for the determination of polysaccharide in Antrodia cinnamonea （AC） was developed.To calculate the total polysaccharide content with the reducing sugar and total sugar content that was detemined by 3,5- dinitrosalicylic acid colorimeitry with glucose as the standard substance. The results showed that： The calibration curve of polysaccharide was in good linearity over the range of 0.25 mg/mL-1.00 mg/mL, the regression equation was y=0.597 9x-0.037 8 （R2=0.999 6）.The average recovery of reducing sugar solution and total sugar solution in the test were 101.65 % with RSD, 1.89 % and 100.92 % with RSD, 2.45 % respectively.The polysaccharide content of AC was 129.5 mg/g-148.1 mg/g.The method which can be used for the quality control of AC is convenient, accurate and sensitive with good reproducibility.
出处
《食品研究与开发》
CAS
北大核心
2016年第9期163-167,共5页
Food Research and Development
基金
深圳市战略新兴产业发展专项资金项目(CYZZ20130416170923767)
关键词
樟芝
多糖
3
5-二硝基水杨酸比色法
A ntrodia cinnamonea
polysaccharide
3,5-dinitrosalicylic acid colorimeitry
