摘要
目的 研究葡萄糖对三角褐指藻细胞生长、岩藻黄素含量和岩藻黄素生物合成相关基因表达的影响。方法 本实验以分光光度法研究葡萄糖对三角褐指藻生长影响,HPLC研究藻细胞岩藻黄素含量,荧光定量聚合酶链式反应（PCR）研究岩藻黄素生物合成相关基因表达。结果 在平台期末,葡萄糖处理组藻细胞密度均比对照组高,说明葡萄糖促进三角褐指藻细胞生长。HPLC结果表明,随着葡萄糖浓度的升高,三角褐指藻细胞中岩藻黄素含量呈逐渐下降的趋势。其中,葡萄糖为50mg·L-1时,岩藻黄素含量最低,为0.26 mg·g-1干重,比对照组降低了67.5%,说明葡萄糖抑制了三角褐指藻岩藻黄素的生物合成。荧光定量PCR结果表明,10-50 mg·L-1葡萄糖处理三角褐指藻时,岩藻黄素生物合成相关基因zep、pys、zds、lcyb、crtiso和pds的表达量均比对照组低。该结果与岩藻黄素含量变化一致。结论 葡萄糖抑制三角褐指藻岩藻黄素的生物合成是通过下调岩藻黄素生物合成相关基因表达来实现的。
OBJECTIVE To explore the effects of glucose on the growth of algae, the content of fucoxanthin, and expressions of genes related to biosynthesis of fucoxanthin. METHODS The cell growth of algae induced by glucose was researched by using spectrophotometric method. The content of fueoxanthin was measured by HPLC. The expressions of＇ the genes which were related to biosyn- thesis of fueoxanthin were detected by using quantitative PCR. RESULTS At the end of the platform period, the density of cells trea- ted by glucose was higher than that of the control group. The growth of P. tricornutum was promoted by glucose. The result of HPLC a- nalysis showed that fucoxanthin content in the algae treated by different concentrations of glucose was decreased than that in the control group. When the concentration of glucose reached 50 mg·L- 1 , the content of fucoxanthin was the lowest （ 0. 26 mg ·g-1· DW ） , which was 67.5% lower than the control, indicating that glucose inhibited the biosynthesis of fucoxanthin in P. tricornutum. RT-qPCR result showed that the expression of genes related to the biosynthesis pathway of fucoxanthin, i.e. , zep, pys, zds, lcyb, crtiso, and pds, were all lower than those of the control when the concentration of glucose was in the range from 10 to 50 mg · L -1. This result was consistent with the change of fucoxanthin content. CONCLUSION This result further illustrates that glucose may inhibit the biosyn- thesis of fucoxanthin in P. tricornutum by down-regulating the expression of related genes.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2016年第14期1230-1234,共5页
Chinese Pharmaceutical Journal
基金
浙江省科技厅重点科技创新团队资助项目(2012R10029-07
2010R50029)
宁波科技攻关项目(2013C10018)
宁波市农村创新创业项目(2014C91023)
浙江省大学生科技创新活动计划暨新苗人才计划资助项目(2014R405050)
宁波大学优秀学位论文培育基金资助项目(py2014020)
