摘要
目的:观察七龙天对低氧性肺动脉高压(HPH)大鼠内皮素-1(ET-1)、血管内皮细胞一氧化氮合成酶(NOS)的影响。方法:雄性SD大鼠随机分为6组:空白对照组(A)、模型组(B)、波生坦组(C)、七龙天低剂量组(D)、七龙天中剂量组(E)、七龙天高剂量组(F)。A组大鼠置于正常环境中饲养,B、C、D、E、F组均造模。采用常压低氧法制模,从制模第1天始给药,连续3周,A、B两组均给予蒸馏水3 m L/(只·d)。在直视下行右心导管术测定肺血流动力学参数,及RT-PCR法检测肺组织中NOS、ET-1 mRNA表达。结果:七龙天及波生坦均可明显降低大鼠肺动脉高压,且以七龙天高剂量组效果为佳,疗效呈药物剂量依赖表现。各组NOS、ET-1表达量与肺动脉压具有相关性,但二者在高剂量七龙天调节至正常范围时,肺动脉压仍未恢复正常水平。结论:不同剂量七龙天均可降低肺动脉高压,且高剂量七龙天疗效优于波生坦。其降压机制与降低NOS及ET-1表达量有关,但仍可能受其他炎症因子及血管新生因子影响。
Objective： To study the effects of Qilongtian on plasma endothelin（ ET- 1） and nitrie oxide synthase（ NOS） in pulmonary arterial hypertension（ PAH） rats. Methods： The HPH model in male SD rats was induced by atmospheric hypoxia. Bosentan was used as positive control drug. The pulmonary hemodynamic parameters were detected by under direct vision. Expression of ET- 1 and mRNA NOS in lung tissues was detected by RT- PCR method. Results： Qilongtian and Bosentan could significantly reduce the levels of mean pulmonary arterialpressure（ MPAP）,ET- 1 and NOS expressions in HPH rats（ P〈0. 05 or P〈0. 01）and the effect of high dose group was better. The expressions of NOS and ET- 1 were correlated with pulmonary arterial pressure,but both of them in the high dose of Qilongtian were adjusted to be normal when pulmonary arterial pressure was not returned to normal level. Conclusion： Qilongtian can effectively reduce MPAP in HPH rats by reducing ET- 1 and NOS contents. Different doses of Qilongtian can reduce the pulmonary artery pressure and the effect of high dose of Qilongtian is better than that of bosentan.Its anti- hypertensive mechanism is related to the decrease of NOS and ET- 1 expressions,but it may still be influenced by other inflammatory factors and angiogenesis factors.
出处
《辽宁中医杂志》
CAS
北大核心
2016年第7期1486-1489,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81160467)
