摘要
为了解绵羊和山羊嗜血支原体在甘肃、辽宁、内蒙古、宁夏、重庆、海南等地的流行状况及病原种类,利用瑞氏染色方法和已发表的嗜血支原体通用qPCR方法,对2011—2015年间采自上述地区的绵羊和山羊血液样品346份进行了检测,并对部分qPCR阳性样品扩增产物进行了测序和序列分析。结果,在甘肃、内蒙古的绵羊样品中和重庆、海南的山羊样品中检出嗜血支原体;其中,瑞氏染色方法的绵羊样品阳性率为12.45%(33/265),山羊样品阳性率为24.69%(20/81);qPCR绵羊阳性率为22.26%(59/265),山羊阳性率为37.04%(30/81)。对部分阳性样品的qPCR产物进行的序列分析结果显示,绵羊样品中5/7份为绵羊支原体和绵羊嗜血支原体待定种共同感染,1/7为绵羊支原体感染,1/7为绵羊嗜血支原体待定种感染;山羊样品中,2/3为绵羊支原体感染,1/3为绵羊嗜血支原体待定种感染。本文在我国首次证实山羊和绵羊中流行绵羊支原体和绵羊嗜血支原体待定种两种不同的嗜血支原体,且绵羊中存在两种嗜血支原体共同感染的情况。
To determine the prevalence of hemoplasmas and to identify the predominant hemoplasma species in sheep and goats in Gansu,Liaoning,Inner Mongolia,Ningxia,Chongqing and Hainan,a total of 346 blood samples collected from these regions between 2011 and 2015 were screened using both Wright stainning of blood smears and a pan-hemoplasma qPCR assay followed by sequencing. The results showed that the hemoplasmas were detected in the sheep blood samples from Gansu and Inner Mongolia,as well as in the goat blood samples from Chongqing and Hainan. 12.45% （33/265）sheep samples and 24.69%（20/81） goat samples were positive for hemoplasma using Wright stainning,respectively. 22.26%（59/265）sheep and 37.04%（30/81） goat samples showed positive for bemoplasma qPCR detection. Sequences analysis indicated that 5 of 7 sheep were cocurrently infected with Mycoplasma ovis and Candidatus Mycoplasma haemovis,one being infected with Mycoplasma oyis only and the other infected with Candidatus Mycoplasma haemovis only. In 3 goat samples,2 were infected with Mycop2asma ovis and one was infected with Candidatus Mycoplasma haemovis. These results confirmed for the first time that both Mycoplasma ovis and Candidatus Mycoplasma haemovis were prevalent in Chinese sheep and goat populations,and co-infection in sheep with the two different hemaplasma species was also dicovered in China for the first time.
出处
《中国兽医科学》
CAS
CSCD
北大核心
2016年第7期821-826,共6页
Chinese Veterinary Science
基金
国家科技支撑计划项目(2015BAD12B02
甘肃省科技支撑计划项目(1204NKCA071)
兰州市城关区科技计划(2012-2-1)
