摘要
背景:基质金属蛋白酶13对软骨细胞外基质中的Ⅱ型胶原蛋白的降解能力最为活跃,白细胞介素1β被认为是基质金属蛋白酶的诱导剂,参与关节软骨的降解和退变。目的:分析白细胞介素1β对大鼠软骨细胞miR-27b和基质金属蛋白酶13蛋白表达的影响。方法:雄性Wistar大鼠7只,体外分离培养软骨细胞,并分组为白细胞介素1β刺激组及对照组。对照组不采取任何刺激;白细胞介素1β刺激组采用10μg/L白细胞介素1β的无血清培养基培养大鼠软骨细胞,于第0,24,48小时分别采用倒置显微镜观察细胞生长情况,并收集细胞,提取RNA检测软骨细胞基质金属蛋白酶13表达以及不同的miR NAs的表达变化。结果与结论:(1)白细胞介素1β诱导大鼠软骨细胞0,24,48 h时基质金属蛋白酶13相对表达量逐渐增加(P<0.05);(2)白细胞介素1β诱导大鼠软骨细胞24,48 h时miR-27b、miR-31表达均逐渐下降(P<0.05);mi R-26a、miR-26b、miR-23和miR-204表达均逐渐加强(P<0.05),白细胞介素1β诱导大鼠软骨细胞48 h后,mi R-27b表达的降低最为显著(P<0.05);(3)结果说明白细胞介素1β诱导大鼠软骨细胞,miR-27b表达显著下降,靶定调控基质金属蛋白酶13的能力降低,导致软骨细胞的损伤,加剧了骨性关节炎的发病及进展。
BACKGROUND：Matrix metal oproteinase-13 is most active in the degradation of col agen type II in the extracel ular matrix of cartilage. Interleukin-1 （IL-1） is thought to be the inducer of matrix metal oproteinases, and participates in the degradation and degeneration of articular cartilage. OBJECTIVE：To study the influence of IL-1βon microRNA-27b （miR-27b） and matrix metal oproteinase-13 expression of chondrocytes in rats. METHODS：Chondrocytes isolated from seven male Wistar rats were cultured and divided into IL-1βstimulation group and control group. No stimulus was given in the control group;10μg/L of serum free medium was used to culture rat chondrocytes in the IL-1βstimulation group. Cel growth was observed at 0, 24, and 48 hours under an inverted microscope. miR-27b and matrix metal oproteinase-13 expression in the cultured chondrocytes were detected. RESULTS AND CONCLUSION：The relative expression of matrix metal oproteinase-13 in rat chondrocytes was gradual y increased when induced by IL-1βat 0, 24, and 48 hours （P〈0.05）. Expression of miR-27b and miR-31 in rat chondrocytes at 24 and 48 hours induced by IL-1βgradual y decreased （P〈0.05）;conversely, expression of MiR-26a, miR-26b, miR-23, and miR-204 gradual y increased （P〈0.05）. After 48 hours of IL-1βinduction, expression of miR-27b was the lowest in rat chondrocytes （P〈0.05）. These findings suggest that IL-1βinhibits miR-27b expression, strengthens the expression of matrix metal oproteinase-13, and damages chondrocytes, contributing to both the onset and progression of osteoarthritis.
出处
《中国组织工程研究》
CAS
北大核心
2016年第29期4277-4283,共7页
Chinese Journal of Tissue Engineering Research
