摘要
背景:细胞纯化方法可以消除细胞的生物变异性,为细胞再生治疗提供了新的思路。目的:探讨CD133+细胞在人脐血单个核细胞移植治疗心力衰竭中的作用。方法:(1)采用淋巴细胞分离液法提取人脐血单个核细胞,经免疫磁珠分离CD133+细胞及CD133-细胞,调整细胞浓度为1×108 L-1;(2)将40只SD大鼠随机分为5组:假手术组、模型组、CD133+细胞组、CD133-细胞组和单个核细胞组,除假手术组,其余4组腹腔注射异丙肾上腺素建立心脏衰竭模型,CD133+细胞组经尾静脉注射1 mL CD133+细胞和1 mL PBS,CD133-细胞组经尾静脉注射1 mL CD133-细胞和1 mL PBS,单个核细胞组经大鼠尾静脉注射1 mL CD133+细胞和1 mL CD133-细胞,假手术组和模型组经尾静脉注射2 mL PBS;(3)细胞移植后4周,观察大鼠的心肌病理组织学改变,心肌纤维化程度以及CD133+细胞在心肌内的定植情况。结果与结论:(1)苏木精-伊红染色结果:模型组大鼠心肌排列紊乱,假手术组心肌排列整齐,CD133+细胞组心肌紊乱程度最轻,单个核细胞组其次,CD133-细胞组和模型组接近;(2)Masson染色结果:模型组胶原纤维增多,排列紊乱,胶原纤维断裂,假手术组胶原纤维很少,排列整齐,CD133+细胞组心肌胶原纤维减少和紊乱程度最轻;(3)模型组、CD133+细胞组、CD133-细胞组和单个核细胞组的胶原纤维面积均明显高于假手术组(P<0.05),其中CD133+细胞组的胶原纤维面积最小;(4)免疫组化和免疫荧光染色结果:在移植后4周各组大鼠心肌组织中均未发现特异性染色细胞;(5)结果表明,CD133+细胞移植治疗可以改善心肌受损程度,减少心肌纤维化程度,其治疗效果较单个核细胞移植治疗效果好,CD133+细胞并未定植于心脏局部。
BACKGROUND：Cel purification can eliminate the biological variability of cel s, providing new insight into cel regeneration therapy. OBJECTIVE：To study the Influence of CD133＋cel s on human umbilical cord blood mononuclear cel transplantation for treatment of heart failure. METHODS：Human cord blood mononuclear cel s were isolated using lymphocyte separation medium method, and CD133＋and CD133-cel s were sorted using immunomagnetic beads at a cel density of 1×108/L. Forty Sprague-Dawley rats were randomized into five groups：sham group, model group, CD133＋cel group, CD133-cel group and mononuclear cel group. Animal models of heart failure were made using intraperitoneal injection of isoproterenol in al the groups except for the sham group. Rats in the CD133＋cel group and CD133-cel group were given 1 mL CD133＋cel s plus 1 mL PBS and 1 mL CD133-cel s plus 1 mL PBS via the tail vein, respectively. Rats in the mononuclear cel group were given 1 mL CD133＋cel s plus 1 mL CD133-cel s via the tail vein, and those in the sham and model groups given 2 mL PBS via the tail vein. After 4 weeks, cardiac pathology, degree of myocardial fibrosis and colonization of CD133＋cel s in myocardial tissues were observed. RESULTS AND CONCLUSION：Hematoxylin-eosin staining showed that myocardial tissues arranged disorderly in the model group, but regularly in the sham group;myocardial disorders were mildest in the CD133＋cel group, successively fol owed by the mononuclear cel group, and severest in the CD133-cel group and model group. Masson staining showed that in the model group, col agen fibers were proliferated, arranged irregularly and even broken, while in the sham group, the col agen fibers were less in number and arranged in order. Additional y, there was less reduction in col agen fibers and milder myocardial disorders in the CD133＋cel group compared with the other groups. Area of col agen fibers was increased significantly in al the groups except for the sham group （P〈0.05）, but this increment was th
出处
《中国组织工程研究》
CAS
北大核心
2016年第28期4196-4202,共7页
Chinese Journal of Tissue Engineering Research
