摘要
目的:探讨蛋白质酪氨酸磷酸酶1B(protein tyrosine phosphatase 1B,PTP1B)对子宫内膜癌细胞增殖和凋亡的影响。方法:体外培养人子宫内膜癌细胞株(Ishikawa),并将其分为4组,即空白对照组(A组),凋亡组[B组,十二烷基硫酸钠(SDS)],空病毒载体凋亡组(C组,SDS+Lacz)和过表达凋亡组(D组,SDS+PTP1B)。利用Western blotting检测PTP1B和Bcl-2表达;四甲基偶氮唑蓝(MTT)检测细胞活力,吖淀橙/溴化乙啶(AO/EB)双染色观察过表达PTP1B对Ishikawa形态学的影响。结果:Western blotting显示Ishikawa无PTP1B表达,D组Ishikawa高表达PTP1B。A组、B组、C组及D组的Ishikawa均有Bcl-2的表达,且D组比B组高37.04%。MMT结果显示D组比B组的细胞存活率提高30.66%。AO/EB结果显示D组仅少量细胞有橙红色荧光,细胞形态较规则。结论:过表达PTP1B能在一定程度上促进子宫内膜癌Ishikawa细胞增殖并抑制其凋亡。
Objective： To investigate the effect of protein tyrosine phosphatase 1B （PTPIB） on the proliferation and apoptosis of endometrial cancer Ishikawa cell line. Methods： Endometrial cancer cell line （Ishikawa） was divided into four groups after in vitro culture： group A （control group）, group B （apoptosis group）, group C （empty virus group） and group D （overexpressed PTP1B group）. Then, the expressions of PTP1B and Bcl-2 were detected by Western blotting, the cell viability was tested by four methyl thiazolyl tetrazolium （MTT）, and the morphology of endometrial cancer Ishikawa cell line was detected by acridine orange/ethidium bromide （AO/EB） staining, respectively. Results： Western blotting analysis showed that PTP1B was expressed in endometrial cancer cells with overexpress of PTP 1 B, without expression in the control endogenous endometrial cancer cells. Bcl-2 was visible for each group. And Bcl-2 protein content of group D increased by 37.04% compared with group B. MMT displayed that cell survival rate of group D raised by 30.66% than that of group B. Also, AO/EB analysis presented that only a few cells appeared orange-red fluorescence in group D, and the cell morphology was normal. Conclusion： PTP1B could improve the proliferation and inhibit the apoptosis of endometrial cancer cells to a certain extent.
出处
《生殖与避孕》
CAS
CSCD
北大核心
2016年第7期542-546,共5页
Reproduction and Contraception
